胰腺的发育专题讲座培训课件.ppt

1、胰腺的发育专题讲座Helena EdlundE8E10胰腺的发育专题讲座2Helena Edlund胰腺的发育专题讲座3Helena Edlund胰腺的发育专题讲座4Micheal German胰腺的发育专题讲座5胰腺的发育专题讲座6胰腺的发育专题讲座7胰腺的发育专题讲座8胰腺的发育专题讲座9胰腺的发育专题讲座10Are pancreatic stem cells unipotent or multipotent?胰腺的发育专题讲座11胰腺的发育专题讲座12胰腺的发育专题讲座13胰腺的发育专题讲座14胰腺的发育专题讲座15胰腺的发育专题讲座16胰腺的发育专题讲座17胰腺的发育专题讲座18胰腺的

2、发育专题讲座19Adult Insulin-and Glucagon-Producing Cells Differentiate From Two Independent Cell Lineages Pedro Luis Herrera Development 127,2000Paper One胰腺的发育专题讲座20Islet Precursor Cell Labeling胰腺的发育专题讲座21Transcriptional STOPBrian Sauer胰腺的发育专题讲座22The Beta Cell Lineage胰腺的发育专题讲座23The Alpha Cell Lineage胰腺的发育

3、专题讲座24Both Alpha and Beta Cells Derive From PDX-1 Expressing Precursors胰腺的发育专题讲座25Direct evidence for the pancreatic lineage:NGN3+cells are islet progenitors and are distinct from duct progenitors Guoqiang Gu,Jolanta Dubauskaite and Douglas A.Melton Development 129,2002Paper Two胰腺的发育专题讲座26Generation

4、 of The Transgenic Mice胰腺的发育专题讲座27PDX1+Progenitors Give Rise to Islets,Acini and Duct in Adult MiceWild TypeHPAP-pdx1-creHPAP+pdx1-cre-ERpGK-creHPAP+pdx1-creLaz/VimentinE9.5/TM 8 week胰腺的发育专题讲座28NGN3+Precursor Cells Give Rise to Isletsngn3-cre胰腺的发育专题讲座29NGN3+cells contribute to the renewal of islets

5、in adult micengn3-cre-ER8 week TMDay 1Day 1Day 7Day 0胰腺的发育专题讲座30Notch Signaling Controls Multiple Steps of Pancreatic Differentiation L.Charles Murtaugh,Ben Z.Stanger,Kristen M.Kwan,and Douglas A.Melton PNAS December 9,2003Paper Three胰腺的发育专题讲座31Notch Signaling and Pancreas Developmentngn3X胰腺的发育专题讲座3

6、2Cre-activated misexpression of activated Notch1胰腺的发育专题讲座33Cre-activated misexpression of activated Notch1E9.5胰腺的发育专题讲座34胰腺的发育专题讲座35E13.5/TM E17.5E11.5/TM E15.5 at E11.5-E13.5胰腺的发育专题讲座36 By Ngn3 PromoterE15.5E13.5胰腺的发育专题讲座37Adult/TMControl胰腺的发育专题讲座38A pulse-chase system for determining the origin of

7、 -cells胰腺的发育专题讲座40Specificity and Dose Dependence of RecombinationHPAP+胰腺的发育专题讲座41Specificity and Dose Dependence of RecombinationHPAP+胰腺的发育专题讲座42-cells are formed by self-duplication of pre-existing -cellsHPAP+Insulin+D 0.14 M1 YD 0.16 M6-8 Week/TM 12 M胰腺的发育专题讲座43-cell Regeneration From Pre-existin

8、g -cells2 weeks after 70%Ppx胰腺的发育专题讲座44Fission of pancreatic islets during postnatal growth of the mouse Philip A.Seymour,William R.Bennett and Jonathan M.W.Slack J.Anatomy 204,2004Paper Five胰腺的发育专题讲座45胰腺的发育专题讲座46胰腺的发育专题讲座47 胰腺的发育专题讲座48 胰腺的发育专题讲座49X-gal staining for-gal activityin the intestinal cryptsHomozygous FemaleHetrozygous Female胰腺的发育专题讲座50Neonatal islets were heterogeneous for-gal expressionGlucagon/-galInsulin/-gal胰腺的发育专题讲座51Dumb-bell islets胰腺的发育专题讲座52StructuralProliferative Units胰腺的发育专题讲座53