1、A Tissue-Like Printed Material -3D打印仿生组织 living tissues Cells communicate with each other tissues display the emergent properties Lipid-coated aqueous droplets join liposomes 连接在一起 form a cohesive,cooperative system 形成协作系统 controlled insertion of specialized embrane proteins 膜蛋白的可控插入research progres
2、s form stable bilayers functionalize with membrane proteins.(Anal.Chem.06)2D light sensors、batteries、simple electrical circuits (Nat.Nanotechnol.09&Anal.Chem 07)release their contents to bulk aqueous solution (nature methods 12&Nat.Nanotechnol.09)BUT.limited to small groups of droplets complexity is
3、 limited3Dstrategy lipid-containing oil含有磷脂分子的油浴eject aqueous droplets Illustration of a printed droplet network.Three characteristics are readily printed communicate through membrane proteins folding driven by differences in osmolarity四个实验验证性质 self-supporting stability membrane proteins are include
4、d in bilayers folding driven by differences in osmolarity1、self-supporting a thermodynamic analysis热力学分析Youngs modulus100 to200 Pa be comparable to living soft tissues2、stability模式图模式图3、membrane proteins are included in bilayers+HL:金黄色葡萄球菌-溶血素 电子通路-HL:绝缘电路Ag/AgCl电极4、folding driven by differences in
5、osmolarity higher and lower osmolarityequalseveral prerequisites for folding:the network must fold slowly as compared to the printing time stress can cause the network to buckle inan uncontrolled manner solutions:printing rate,salt concentrations,droplet size,adjustments to the network geometrymore than 3 hoursMovie S1Movie S2应用：to deliver drugs upon a specific signa tissue engineering.3D生物打印的发展十分迅速，近些年相继报道多项研究成果。
