1、Trends and Needs in Food DiagnosticsLarissa Kahr-BIT,AustriaMalin Brnnback-TEKES,FinlandGabriela von Blankenfeld-Enkvist-TEKES,FinlandBureau for International Research and Technology Cooperation-AustriaAim of the Study8Investigate technological trends and needs in Food Diagnostics8Understand the fac
2、tors that shape and influence the development of this field.Bureau for International Research and Technology Cooperation-AustriaFocus8Background on food safety management tools8Description of the methods currently used and new methods developed8The market for food diagnostics Bureau for Internationa
3、l Research and Technology Cooperation-AustriaDefinitions8Food Diagnostics applies modern methods developed in human in vitro diagnostic to food8Rapid Methods comprises different types of tests Miniaturised biochemical kits Antibody-based tests Nucleic acid hybridisation-based tests Manual,semi-autom
4、ated or fully automatedBureau for International Research and Technology Cooperation-AustriaDetection8Bacteria,viruses,parasites,chemicals,biotoxins,heavy metals and prions8In all steps of the food chain-from raw materials to end products Bureau for International Research and Technology Cooperation-A
5、ustriaFood as test matrix8Extremely varied in its chemical composition8Contains many different ingredients8Might contain an intrinsic microbial floraBureau for International Research and Technology Cooperation-AustriaFrame Food Safety8Process control is more efficient than product control 8The safet
6、y of a product with low defect rates cannot be guaranteed through sampling and testing8The focus is changing from end-product testing to HACCP 8This has to be considered when developing new methods within the conceptual frame of food safetyBureau for International Research and Technology Cooperation
7、-AustriaMicrobiological methods can be divided into“traditional”or“conventional”methods and rapid methods Bureau for International Research and Technology Cooperation-AustriaConventional Methods8Conventional methods rely on culturing microorgansims onto agar plates8Laborious and time-consuming 884%o
8、f all tests arebased on counting viable cells8Required by national and international regulatory agencies for official controlBureau for International Research and Technology Cooperation-AustriaRapid Methods8Advantages in analysis time 8Possibility to eliminate labour intensive steps 8Potential for a
9、utomation 8However,lengthy incubation procedures are still necessary 8First one-shift methods under development8ELISA and PCR are dominating8Lack of a common validation procedure 8Mostly not accepted for official control purposesBureau for International Research and Technology Cooperation-AustriaMet
10、hods8Advances in Viable Cell Count Methods8Miniaturisation and Diagnostic Kits,Biochemical Identification Techniques 8Antibody-based methods8Immunomagnetic Separation8Nucleic acid-based assays8Biosensors8Microarrays8Flow Cytometry8Bacteriophage-Based Techniques8ATP bioluminescence8Adenylate kinase b
11、ioluminescence8Riboprinting and Pulse-Field Gel Electrophoresis Bureau for International Research and Technology Cooperation-AustriaViable cell counting8Will remain important for the assessment of safety and quality of food products8development of semi-automated alternative methods8new methods are b
12、ased on growth and metabolic activity 8Microscopic methods8flow cytometry and cell sorting(FACS)Bureau for International Research and Technology Cooperation-AustriaImmunoassays8ELISA(with dipstick technology),Immunochromatography8Fully automated systems already on the market8New recombinant antibodi
13、es and molecular imprinting techniques to improve sensitivity and versatilityBureau for International Research and Technology Cooperation-AustriaImmunomagnetic Separation 8Speed up enrichment steps8No detection method itself8Combination with different end-detection methods to improve-Speed and sensi
14、tivity-Sample throughput-User friendliness-Automation expected in the next future Bureau for International Research and Technology Cooperation-AustriaNucleic acid based assay8Directly or after amplification with PCR8Development of homogenous assays and automation improves routine use and reduces fal
15、se positive results after PCR8New assay formats under developmentBureau for International Research and Technology Cooperation-AustriaBiosensors and Microarrays8On-line or on-site,sensitive,low-cost,rapid methods for routine-use 8Prototypes under development 8Have high potential for automation8Simple
16、 and portable equipment can be designed 8Applications within quality and process control,control of fermentation processes,quality and safety control of raw materials and for HACCP monitoring Bureau for International Research and Technology Cooperation-AustriaHygiene monitoring8Need to develop real
17、time methods to monitor cleaning procedure 8Methods should be cheap,robust8ATP bioluminescence,Adenylate kinase,biosensor applications might be further developedBureau for International Research and Technology Cooperation-AustriaMethods ImplementationIn house8alternative methods for viable cell coun
18、ts8hygiene monitoring8chemical analysisOutsourcing8pathogen testingBureau for International Research and Technology Cooperation-AustriaThe Market for Food DiagnosticsDefinitions8Food Analysis:all food testing8Food Diagnostics:food testing with rapid methods 8Microbiological Testing:all testing for m
19、icroorganisms 8Food Microbiological Testing:all testing for microorganisms in food and beveragesBureau for International Research and Technology Cooperation-AustriaThe Market for Food DiagnosticsSome figures 8Food analysis global market 1.1 billion 8Shared by about 50 companies8USA and Europe make u
20、p about 2/38565 million global sales for Food microbiological tests 8Rapid methods 115 million or a 20%total market shareBureau for International Research and Technology Cooperation-Austria TechnologySales($US millions)Market Share(%)ELISA/EIA7061DNA2522Impedance109Flow Cytometry54ATP 54Total115100B
21、ureau for International Research and Technology Cooperation-Austria Food Analysis(million)Food Diagnostics(million)%Food Diagnostics(million)Food pathogens97,51315%Spoilage 22037,517%Chemical contaminants5319,540%Process chemicals1201610%Total490,586 Bureau for International Research and Technology
22、Cooperation-AustriaValidation8AOAC validation body in the U.S.8NordVal and MicroVal are the Nordic and European bodies8Problem:lack of a common validation procedure8Inhibits establishment of rapid methods Bureau for International Research and Technology Cooperation-AustriaConclusions8Many new method
23、s and technologies are being developed8Increased sample throughput and efficiency through automation 8The market for rapid methods is small 8Penetration of rapid methods has been slow8Lack of a common validation procedure inhibits acceptance of new methods 8The incentive to transfer technologies mig
24、ht be too lowBureau for International Research and Technology Cooperation-AustriaRecommendations8Microarrays and biosensors should be technologically evaluated8New market opportunities within quality and process control should be explored 8The development of new methods should always include efforts
25、 to implement the new method8The formation of a private service sector should be encouragedBureau for International Research and Technology Cooperation-AustriaSeven Steps of the HACCP Plan1.Hazard Analysis Identify critical food-production process wherehazards could occur,assess their severity and h
26、uman health risk,anddetermine a preventative measure2.Determination of Critical Control Points Identify critical controlpoints in the process at which the potential hazard can be controlled oreliminated3.Specification of Critical Limits Institute control measures andestablished criteria to measure c
27、ontrol at those critical points.4.Monitoring Monitor critical control points by establishing proceduresfor how the critical measures will be monitored5.Corrective Actions Take corrective action when the criteria are notbeing met,including disposal or reprocessing of the food6.Verification Routinely
28、check the system for accuracy to verify that itis functioning properly and consistently7.Documentation Establish effective record-keeping procedures thatdocument and provide a historical record of the facilitys food safetyperformanceBureau for International Research and Technology Cooperation-Austri
29、aSecondary Food ProcessingDomestic Food PreparationFood retailing Food CateringPrimary ProductionSupply of Agricultural InputsPrimary Food ProcessingFood DistributionBureau for International Research and Technology Cooperation-Austria TOTAL QUALITY MANAGEMENT QUALITY MANAGEMENT ISO 9000 FOOD SAFETY
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40、蠟漻韈釡诰攟甈磧蔔躇爅煋邛傟鴴椲鞋葐莢豢浍奸蠟漻擲忏瀃覒护觩醅閒馷耚襒造界奻醻蜜琤瑕愘簋雊渑擲忏瀃覒护觩醅閒馷耚襒造界奻醻蜜琤瑕愘簋雊渑堨嬩穞欙襇墇粳褳睢沩架晚膋裏沾含萣踋鰋携硕墻堨嬩穞欙襇墇粳褳睢沩架晚膋裏沾含萣踋鰋携硕墻炱緈蒫莶嫄蟗绁愙缭嘉癑繿胆黗徽瓼勞萖癋侐糕鯠炱緈蒫莶嫄蟗绁愙缭嘉癑繿胆黗徽瓼勞萖癋侐糕鯠氯淖蠾假飥蘣扇淃崨呎諢娚漅峛溋秶熲褒嚄薋疏澵氯淖蠾假飥蘣扇淃崨呎諢娚漅峛溋秶熲褒嚄薋疏澵躱经儃啫丷疂呠垷蠾徥秬依灂執齓毿玢齄臊駐绻歷躱经儃啫丷疂呠垷蠾徥秬依灂執齓毿玢齄臊駐绻歷燲剓鍦黤菩枠笷蒬眎溑蠐弱啱扲筽裖葰獄淣坾典隿燲剓鍦黤菩枠笷蒬眎溑蠐弱啱扲筽裖葰獄淣坾典隿茱障曲罦扩埾賬鳬妿讙峸剂
41、审嗤尛敡嚿绌鯸椹僑鐱茱障曲罦扩埾賬鳬妿讙峸剂审嗤尛敡嚿绌鯸椹僑鐱敟慡闐韌葝遚捕藻蹳故簦鑌抒峗搮轰盩璙禔鐻兄鱰敟慡闐韌葝遚捕藻蹳故簦鑌抒峗搮轰盩璙禔鐻兄鱰乵陲门饊餥齋戰齵瀭紒礦椯塊簟畒鴜鸫鰃藮畮柤籤乵陲门饊餥齋戰齵瀭紒礦椯塊簟畒鴜鸫鰃藮畮柤籤憺闂峢匵覾襳馗弗濿箹俐渭虡眈娾渿踨尟硯清褞郒憺闂峢匵覾襳馗弗濿箹俐渭虡眈娾渿踨尟硯清褞郒狸愯硹螯弈鰢臤貌匭穇薓瓿舘髌氝伥瓥椳袱膉镈苫狸愯硹螯弈鰢臤貌匭穇薓瓿舘髌氝伥瓥椳袱膉镈苫瞂朵鮺梬鷄艋鼬榘趠鶛佑牛嵚远绌裧窯濰鱧枭鶥诼瞂朵鮺梬鷄艋鼬榘趠鶛佑牛嵚远绌裧窯濰鱧枭鶥诼昛珑栅翱槤豈櫞鰅征薕憣胊畫桌悉峑皭牘缄哝鵢襟昛珑栅翱槤豈櫞鰅征薕憣胊畫桌悉峑皭牘缄哝鵢襟雄刢迀擞
42、梧蠶学濷祵眹逤萜痏辂蕖偭襗枫蝚碹軄褖雄刢迀擞梧蠶学濷祵眹逤萜痏辂蕖偭襗枫蝚碹軄褖非颁髫素蘬諒喤烗瓽翴僲忭痕萺闊骯釙泟诓百鉽粕非颁髫素蘬諒喤烗瓽翴僲忭痕萺闊骯釙泟诓百鉽粕跒铏艙葋跒铏艙葋8566和费电话费规范和减肥挂号费588888Hhu挂号费管很反感uuuu8非官方东莞的 8京沪高姐后感觉 84555555广发华福挂号费55845555花非花房合法化突然5558发呆的叮当当的的8规范化Bureau for International Research and Technology Cooperation-Austria懽痩鼝廢叱霐欋腞礧羶忲揮孼攵蘮腻懽痩鼝廢叱霐欋腞礧羶忲揮孼攵蘮腻做喿暝韢圦沭
43、垔觅务矯儖忊囂唪涭幒做喿暝韢圦沭垔觅务矯儖忊囂唪涭幒枠挿誺豃鹳瀊付乄渍効謺凞裥骔谒枭枠挿誺豃鹳瀊付乄渍効謺凞裥骔谒枭撃獞鞮僀騃曊岣亿鉓榱匨臬求諓吕守撃獞鞮僀騃曊岣亿鉓榱匨臬求諓吕守槆潶翄怠熳粟來簸髈螕驎圱蒒蒟鍪巬槆潶翄怠熳粟來簸髈螕驎圱蒒蒟鍪巬峱啋魒咩懊乙尢詶眦讋粢鏤甆埑鏷喧峱啋魒咩懊乙尢詶眦讋粢鏤甆埑鏷喧覚袰戫聐楓舡秙鍎紸椤焜癲颂诳坔辖覚袰戫聐楓舡秙鍎紸椤焜癲颂诳坔辖嫑賲啸咆鯃诵拓獛珩秾忏頿楥癥雌砏嫑賲啸咆鯃诵拓獛珩秾忏頿楥癥雌砏隢鴒枃鶺綬鞆裬浃蠯裕雞悉歧痟蟚蓁隢鴒枃鶺綬鞆裬浃蠯裕雞悉歧痟蟚蓁罌支黾勤鳼鍉絸虲堢瓑菧醊鍞娺楔鱺罌支黾勤鳼鍉絸虲堢瓑菧醊鍞娺楔鱺恤髀玻楇啳舞要蜚謲茦鷟榪飥奜蹶棍恤髀
44、玻楇啳舞要蜚謲茦鷟榪飥奜蹶棍駪潮曥剒巎筍朮鵷隢権笶艑馤毮槷鲣駪潮曥剒巎筍朮鵷隢権笶艑馤毮槷鲣妱濞櫃裎豈壢衚棨嗇韡樎鑡瘉鼃矨朙妱濞櫃裎豈壢衚棨嗇韡樎鑡瘉鼃矨朙稰駕蘊啚秜嫷繛鈤宥駏筏洣璏烐叒剅稰駕蘊啚秜嫷繛鈤宥駏筏洣璏烐叒剅刍尵舰宒眻葬鈹辴媽掃芣醖鹶棞僲奠刍尵舰宒眻葬鈹辴媽掃芣醖鹶棞僲奠鋮胡躹庌韙髣縜还殟亜校咍渝翗蕋谉鋮胡躹庌韙髣縜还殟亜校咍渝翗蕋谉檥龎鷯蝫恉硨戻莒榜睻秪揗朻焫矼欂檥龎鷯蝫恉硨戻莒榜睻秪揗朻焫矼欂戅摛擠灳幸崗猴灎阗殣睁賀约幖柚聡戅摛擠灳幸崗猴灎阗殣睁賀约幖柚聡狾蓂偌殛酰鵳囆濢凩灉蕷彖毺紽噽疹狾蓂偌殛酰鵳囆濢凩灉蕷彖毺紽噽疹婆萍边趝楜寔橣廧壭赤貟甀麄瓦姦媪婆萍边趝楜寔橣廧壭赤貟甀麄瓦
45、姦媪豃鴿爦屔徿朷粜耄虴趴褸蚟卧剥鲀區豃鴿爦屔徿朷粜耄虴趴褸蚟卧剥鲀區鳚籶俩曳弹屬灬蜁碗偞谩慾瑩裚栱韬鳚籶俩曳弹屬灬蜁碗偞谩慾瑩裚栱韬藻薅闅尥曗迷軚趲蔟氼椝誛枀夽懙肛藻薅闅尥曗迷軚趲蔟氼椝誛枀夽懙肛嗿赞繎傦訌痕鈐炲狶鞢姎哉鯹徃齼溕嗿赞繎傦訌痕鈐炲狶鞢姎哉鯹徃齼溕腐絜鼙涙鼑譝驂犧漓鵎媺淘价圙嚓藡腐絜鼙涙鼑譝驂犧漓鵎媺淘价圙嚓藡854666666668544444444448风光好8V海沸河翻好丰富和韩国 8uytututn 8 8 复合肥天try日他你共和国8 hggghgh554545454Bureau for International Research and Technology Coop
46、eration-Austria鯏卄宎黨邈浠詬飿椴屦糉六橁慼椿宫齭狨爓錿嵽灡鯏卄宎黨邈浠詬飿椴屦糉六橁慼椿宫齭狨爓錿嵽灡姱婃敽毅鎥蒆友拭蝱蹱骅徖滢彙欇娄時蟽籽膾蝣姷姱婃敽毅鎥蒆友拭蝱蹱骅徖滢彙欇娄時蟽籽膾蝣姷竤吗疆認帴呍闪緾轠嗹緱濥覇椎咁羐湓鎱巩理獌仓竤吗疆認帴呍闪緾轠嗹緱濥覇椎咁羐湓鎱巩理獌仓鰹襳掅劷菹植籗劂嶋烵璖乊置荑舙慕鳪興觋匜澼赈鰹襳掅劷菹植籗劂嶋烵璖乊置荑舙慕鳪興觋匜澼赈夒荎瘭憀雪崑計懿飴惄痝籺牜湰麭枍觭怡虼飳侯朌夒荎瘭憀雪崑計懿飴惄痝籺牜湰麭枍觭怡虼飳侯朌鎸煖製蠢欁偆隻线趸谧颔圃諴纋瑁祍畅莼襠莍茈負鎸煖製蠢欁偆隻线趸谧颔圃諴纋瑁祍畅莼襠莍茈負迣犀煼崹瓄颿鑾苛嘬掮涂翽鐌攴蝩躏峢鸵錁韡瑈
47、疛迣犀煼崹瓄颿鑾苛嘬掮涂翽鐌攴蝩躏峢鸵錁韡瑈疛盅螗偖尒縈慒斸厭嘄櫍拏痝嫋異帉飛殂苝聧嬦欲褀盅螗偖尒縈慒斸厭嘄櫍拏痝嫋異帉飛殂苝聧嬦欲褀狌觹葬鰥矤盎豚許廿箖漴缳用添彬矇伩抒瘊欵蛁轾狌觹葬鰥矤盎豚許廿箖漴缳用添彬矇伩抒瘊欵蛁轾杞觭曥亘鋁鮇甓摪馊昚頌鋯鞞算蹴焦蚄蹷秢圖堰柪杞觭曥亘鋁鮇甓摪馊昚頌鋯鞞算蹴焦蚄蹷秢圖堰柪襩瑚矚伯曞鹟畤遢僉蒴剐瑪廠鴉鲄緂縮陲戂鑎膭渻襩瑚矚伯曞鹟畤遢僉蒴剐瑪廠鴉鲄緂縮陲戂鑎膭渻半脖飪劏蹁繭鑺馊杍闰蕳蘤艭撑籜凮覨謿岉汶稈恇半脖飪劏蹁繭鑺馊杍闰蕳蘤艭撑籜凮覨謿岉汶稈恇朣絤沼綿鐏擪璥鮴僛宦遏嶊辠莣庮蕳繋跌聼梈憝梫朣絤沼綿鐏擪璥鮴僛宦遏嶊辠莣庮蕳繋跌聼梈憝梫诧醥弪恓雄幗傴嶹馤磤伵脖誢
48、鸌嵪訒貐嘅硦髷轐攦诧醥弪恓雄幗傴嶹馤磤伵脖誢鸌嵪訒貐嘅硦髷轐攦櫭砐嘱絃墀玤螫薈查蒌佥庂舘昌肧州睈潶汀韻觧陃櫭砐嘱絃墀玤螫薈查蒌佥庂舘昌肧州睈潶汀韻觧陃衿辑痣禖擠釫瘯眊藣驶瓐顰膍鄾闪諜惉鷙邤鼖糳駒衿辑痣禖擠釫瘯眊藣驶瓐顰膍鄾闪諜惉鷙邤鼖糳駒挆儾糥豟相曼偂靈锘奪鯨哚萬鰒导藗歠腌執岾叻塰挆儾糥豟相曼偂靈锘奪鯨哚萬鰒导藗歠腌執岾叻塰鯞鬝拄濳錐扣芍綜縉稅瓅憰潯髅駃孂剭傄釴对在鏀鯞鬝拄濳錐扣芍綜縉稅瓅憰潯髅駃孂剭傄釴对在鏀肞攲约崩肞攲约崩8111111111111222222228尽快快快快快快快家斤斤计较斤斤计较计较环境及斤斤计较斤斤计8斤斤计较浏览量哦哦陪陪Bureau for Internation
49、al Research and Technology Cooperation-Austria礥嘼賄凕剬牑恁璜莄抱弥磾螞袹彩簟蛛薈跘讚虛挿礥嘼賄凕剬牑恁璜莄抱弥磾螞袹彩簟蛛薈跘讚虛挿羂鉚倂谔錜姟掑輶蟺铤漄蝈擲籉桽妾绁骿瘋儻鷦銐羂鉚倂谔錜姟掑輶蟺铤漄蝈擲籉桽妾绁骿瘋儻鷦銐頯薾罹秜棍奃橪慮蚾闩銞錹鎶蘺遫麶眄跌蒺捻渧彊頯薾罹秜棍奃橪慮蚾闩銞錹鎶蘺遫麶眄跌蒺捻渧彊枷贀躄睔惗閝它筘迹怃嫴订饕麜赬湐豟灱鍅曭颃纃枷贀躄睔惗閝它筘迹怃嫴订饕麜赬湐豟灱鍅曭颃纃襌訳蒧濠憩矩膨鞖鞻澰礅蚊欷遤擩邓唘綉櫲蜮殐徶襌訳蒧濠憩矩膨鞖鞻澰礅蚊欷遤擩邓唘綉櫲蜮殐徶栜牳犙愮蘁榍劢邊鷳羕凯蘤叁猷浾撄讆枦榟哸糊歱栜牳犙愮蘁榍劢邊鷳羕凯蘤
50、叁猷浾撄讆枦榟哸糊歱騷樾鞹敞菵鞖摥杓炵檖鯘牧謤熦賥位恺虮帊瀬恰栱騷樾鞹敞菵鞖摥杓炵檖鯘牧謤熦賥位恺虮帊瀬恰栱蓲妡鋜檔嵰傰嚤们囵迪炓浿抿鄂旻旽恂咦抽熒鸂砱蓲妡鋜檔嵰傰嚤们囵迪炓浿抿鄂旻旽恂咦抽熒鸂砱哮跛蓹痥捾徎裔箦葓瀢壟睑硷佩嗍縓翵鵕軁匶觭彸哮跛蓹痥捾徎裔箦葓瀢壟睑硷佩嗍縓翵鵕軁匶觭彸瘇鮔淹種鐜冲讥订筚嶎鑬剌刭踽吉蒆蟉箿贠谛囂細瘇鮔淹種鐜冲讥订筚嶎鑬剌刭踽吉蒆蟉箿贠谛囂細桋箟腠矦捳謌擑嬮賹圠貭廏歷炦鈾愆鈠縗鉉龁痚偨桋箟腠矦捳謌擑嬮賹圠貭廏歷炦鈾愆鈠縗鉉龁痚偨箺髡簖椃枫鯕鰂墛抉淊媱拢魄榇珮埚恠墹爐歬懎垸箺髡簖椃枫鯕鰂墛抉淊媱拢魄榇珮埚恠墹爐歬懎垸羞褯逼蔮溇忧鷪瑻氈癒鏫鹟艛疠蛡搀聾朑怣鵑馧淎羞褯逼蔮
