1、Bioscience Reading and Writing in English,Wei SHI Jilin University QQ群：368834154 （生科院专业外语课） E.mail: shiw59 Tel. 18686638903,Introduction Arrangement How to read paper? How to prepare research? Stem cell research DVD riew,Introduction,What is English? Why we must learn English?,What is English? It is
2、 only a tool. Why we must learn English?,What is English? It is only a tool. Why we must learn English?,What is English? It is only a tool. Why we must learn English? For science level,信息交流文献是的媒体、语言是工具,语言在民族文化发展的贡献与冲突（例）： 印度：殖民统治/英文对社会的影响； 日本：对外来文化的态度拿来主义； 非洲：民族文化的萎缩； 中国：英文会影响我们的传统文化吗？,Lecture Arran
3、gement,Book Teachers Course,生物学英语与写作,于湘晖 吴永革 刘永新 李青山 编,How to read paper？,Intensive reading and extensive reading,The method for faster reading,Reading more and more （to make a nice custom for reading english article every day） Vocabulary No translation,Title Abstract Background Results Discussion,T
4、itle Abstract Background Results Discussion,Title,Understanding 80% from title,Cloning of the variable region genes from hybridoma against HAAH and then construction and expression of anti-HAAH scFv.,Cloning of the variable region genes from hybridoma against HAAH and then construction and expressio
5、n of anti-HAAH scFv.,Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 2010 May;26(5):467-70. Cloning of the variable region genes from hybridoma against HAAH and then construction and expression of anti-HAAH scFv. Article in Chinese Wang H, Xue XP, Lei YF, Song K, Hu YT, Wang W, Yang H. Faculty of Life Sciences
6、, Northwestern Polytechnical University, Xian 710072, China. Abstract AIM: Construction and expression of anti-HAAH single chain variable fragment (scFv) by cloning of the variable region genes from anti-HAAH hybridoma cells G3/F11. METHODS: Total RNA was extracted from hybridoma cells G3/F11. By RT
7、-PCR, murine V(H); and V(L); genes of mAb were amplified respectively. Then, They were assembled into V(H);-linker-V(L); scFv template by SOE-PCR and anti-HAAH scFv was express in E.coli by constructed pHEN 1-Anti-HAAH vector. The expression of Anti-HAAH scFv were detected by SDS-PAGE and Western bl
8、otting and the binding activity were demonstrated by ELISA. RESULTS: The analysis of DNA sequencing shown that the full-length of constructed scFv gene was 744 bp, encoding 248 amino acids. Moreover, the V(H); and V(L); genes were functional antibody variable region genes, as there were four FRs and
9、 three CDRs in both of them. By SDS-PAGE and Western blotting, the expression level of Anti-HAAH scFv were detected. The expression level of pHEN 1-Anti-HAAH scFv, which was expressed in E.coli HB2151, was 7.8% in total E.coli protein and were existed in soluble protein mainly. By indirect ELISA det
10、cetion with HAAH protein, the binding activity of soluble anti-HAAH scFv was very well. CONCLUSION: The murine V(H); and V(L); genes of mAb against HAAH have been cloned successfully and anti-HAAH scFv have been constructed and expressed. Besides, the scFv could be further studied about their biolog
11、ical activity and application, due to their high affinity shown in preliminary detection.,Preparation of monoclonal antibodies against HN protein of ClassI Newcastle disease virus virulent strain.,Preparation of monoclonal antibodies against HN protein of ClassI Newcastle disease virus virulent stra
12、in.,Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 2010 May;26(5):464-6. Preparation of monoclonal antibodies against HN protein of ClassI Newcastle disease virus virulent strain. Article in Chinese Sun YJ, Chen HJ, Song CP, Yu Y, Qiu XS, Yu SQ, Ding C. Shanghai Veterinary Research Institute, Chinese Academy
13、of Agricultural Sciences, Shanghai 200241, China. Abstract AIM: To prepare monoclonal antibodies (mAb) against HN protein of Class I Newcaslte disease virus. METHODS: Several 6-week-old mice were immunized with ClassI 9a5b virulent strain. Hemagglutination inhibition (HI), indirect immunofluorescenc
14、e assay (IFA), cell neutralization test, ELISA and Western blot analysis were used to characterize and identify these antibodies. RESULTS: Four hybridoma cell lines were successfully prepared, designated as mAb 2F5, 2F12, 3H7 and 3H9. Among them, only mAb 3H7 and 3H9 recognized HN protein of the Cla
15、ssI strain, specifically neutralizing NDV and inhibiting hemagglutination. CONCLUSION: mAb 3H7 and 3H9 could be of use in identification of Class I and Class II strains, and in functional studies of HN and cell receptors for NDV. PMID: 20423654 PubMed - in process,EGFR targeting drugs in the treatme
16、nt of head and neck squamous cell carcinoma.,EGFR targeting drugs in the treatment of head and neck squamous cell carcinoma.,Expert Opin Emerg Drugs. 2010 Apr 23. Epub ahead of print EGFR targeting drugs in the treatment of head and neck squamous cell carcinoma. Sundvall M, Karrila A, Nordberg J, Gr
17、nman R, Elenius K. University of Turku, Department of Medical Biochemistry and Genetics, MediCity Research Laboratories, FIN-20520 Turku, Finland. Abstract Importance of the field: Head and neck cancer is the sixth most common cancer worldwide. Despite intense efforts to improve different treatment
18、modalities, mortality rates in advanced cases remain high. Areas covered in the review: EGFR targeting mAb cetuximab (Erbitux) has been approved for the treatment of locally advanced head and neck squamous cell carcinoma (HNSCC) in combination with radiotherapy and for recurrent or metastatic HNSCC.
19、 Here, we review recent scientific advances, as well as future research goals regarding EGFR inhibitors in the treatment of HNSCC. Information was compiled by searching the PubMed, Web of Knowledge and American Society of Clinical Oncology databases for articles published before October 2009. The se
20、arch terms included head and neck cancer, EGFR, cetuximab, panitumumab, zalutumumab, nimotuzumab, erlotinib, gefitinib and lapatinib. The National Institutes of Health registry of clinical trials ( www.clinicaltrials.gov ) was used to search for clinical trials in HNSCC. What the reader will gain: T
21、he background scientific rationale, clinical efficacy and development of EGFR inhibitors in HNSCC are discussed. Take home message: Cetuximab significantly improves survival of patients with locally advanced or metastatic HNSCC. Treatment strategies combining EGFR inhibitors with multimodality appro
22、aches may eventually increase cure rate in HNSCC.,Reading Abstracts,Abstract OBJECTIVE: Antigen-specific therapy targeting selective inhibition of autoreactive responses holds promise for controlling multiple sclerosis (MS) without disturbing homeostasis of the whole immune system. Key autoantigens
23、in MS include myelin proteins, such as myelin basic protein (MBP), proteolipid protein (PLP), and myelin oligodendrocyte glycoprotein (MOG). In this study, we examined the effect of transdermal therapy with myelin peptides on immune responses in the skin, lymph nodes, and peripheral blood immune cel
24、ls of MS patients. METHODS: In a 1-year placebo-controlled study, 30 patients with relapsing-remitting MS were treated transdermally with a mixture of 3 myelin peptides: MBP85-99, PLP139-151, and MOG35-55, or placebo. The phenotype of immune cells in the skin was assessed using immunohistochemistry.
25、 Cell populations in lymph nodes were analyzed using flow cytometry. In peripheral blood immune cells, cytokine production was measured by enzyme-linked immunosorbent assay, and myelin-specific proliferation was examined by carboxyfluorescein succinimidyl ester-based assay. RESULTS: We found that my
26、elin peptides applied transdermally to MS patients activated dendritic Langerhans cells in the skin at the site of immunization and induced a unique population of granular dendritic cells in local lymph nodes. In the periphery, transdermal immunization with myelin peptides resulted in the generation
27、 of type 1, interleukin-10-producing regulatory T cells, suppression of specific autoreactive proliferative responses, and suppression of interferon- and transforming growth factor- production.,Abstract OBJECTIVE: Antigen-specific therapy targeting selective inhibition of autoreactive responses hold
28、s promise for controlling multiple sclerosis (MS) without disturbing homeostasis of the whole immune system. Key autoantigens in MS include myelin proteins, such as myelin basic protein (MBP), proteolipid protein (PLP), and myelin oligodendrocyte glycoprotein (MOG). In this study, we examined the ef
29、fect of transdermal therapy with myelin peptides on immune responses in the skin, lymph nodes, and peripheral blood immune cells of MS patients. METHODS: In a 1-year placebo-controlled study, 30 patients with relapsing-remitting MS were treated transdermally with a mixture of 3 myelin peptides: MBP8
30、5-99, PLP139-151, and MOG35-55, or placebo. The phenotype of immune cells in the skin was assessed using immunohistochemistry. Cell populations in lymph nodes were analyzed using flow cytometry. In peripheral blood immune cells, cytokine production was measured by enzyme-linked immunosorbent assay,
31、and myelin-specific proliferation was examined by carboxyfluorescein succinimidyl ester-based assay. RESULTS: We found that myelin peptides applied transdermally to MS patients activated dendritic Langerhans cells in the skin at the site of immunization and induced a unique population of granular de
32、ndritic cells in local lymph nodes. In the periphery, transdermal immunization with myelin peptides resulted in the generation of type 1, interleukin-10-producing regulatory T cells, suppression of specific autoreactive proliferative responses, and suppression of interferon- and transforming growth
33、factor- production.,Immune regulation of multiple sclerosis by transdermally applied myelin peptides,Abstract Human epidermal growth factor (hEGF) induces the proliferation, differentiation and survival of various cell types including tumor-derived cells. Generally, hEGF performs its biological func
34、tion by binding to a specific receptor (hEGFR) on the cell surface, thereby inducing signal transduction. Suramin, a polysulfonated naphthylurea that acts as a growth factor blocker, exhibits antiproliferative activity against non-small cell lung cancer (NSCLC) cells that overexpress EGFR on the cel
35、l surface. We determined the solution structure of hEGF under physiological conditions and investigated the interaction of suramin with hEGF using isothermal titration calorimetry and NMR spectroscopy techniques. The solution structure of hEGF presented in this paper is different from the bound form
36、 of hEGF present in the crystal structure of the 2:2 EGF-EGFR complex because its C-tail contains a hydrophobic core. This conformational difference supports the hypothesis that hEGF undergoes a conformational change when it binds to hEGFR and subsequently induces signal transduction. Based on the d
37、ocking structure of the hEGF-suramin complex, we demonstrated how suramin blocks hEGF by binding to its receptor binding site (the C-terminal region around Arg45) and inhibits the crucial conformational change.,The NMR solution structure of human epidermal growth factor (hEGF) at physiological pH an
38、d its interactions with suramin.,Abstract An ultrasensitive fluorescence immunoassay method for quantitative detection of single molecules is developed on the basis of counting single magnetic nanobeads (MNBs) with combined amplification of DNA and dye/DNA conjugate. Highly amplified fluorescence si
39、gnal and low background signal are achieved by using mutilabel bioconjugates made by linking multiple dye/DNA conjugates to streptavidin-coated magnetic nanobeads (SA-MNBs) and magnetic separation. In this method, human IgG (Ag) is captured on the silanized glass substrate surface, followed by immun
40、oreaction with biotinylated mouse antihuman antibody (BT-Ab). Then, SA-MNBs are attached to the BT-Ab through the biotin/streptavidin interaction at a ratio of 1:1. Subsequently, a 30 base pair double-stranded oligonucleotide terminated with biotin (BT-dsDNA) is conjugated to the SA-MNBs. The result
41、ant Ag-BT-Ab-SA-MNBs/BT-dsDNA/SYBR Green I is achieved after a fluorescent DNA probe, SYBR Green I, is added to the substrate and bound to the oligonucleotide at high ratios. Finally, epifluorescence microscopy coupled with a high-sensitivity electron multiplying charge-coupled device is employed fo
42、r human IgG fluorescence imaging and detection. The number of fluorescent spots corresponding to single protein molecules on the images is counted. It is found that the number of fluorescent spots resulting from the SA-MNBs/BT-dsDNA/SYBR Green I immuotargeted on the glass slides is correlated with t
43、he concentration of human IgG target antigen in the range 3.0-50 fM.,Quantitative Detection of Single Molecules Using Enhancement of Dye/DNA Conjugate-Labeled Nanoparticles,Abstract We describe here a protocol to faithfully amplify global cDNAs from single cells. The amplified cDNAs retain their sen
44、se-antisense orientation and can be easily applied to template preparation for quantitative high-density oligonucleotide microarray analyses. The amplification protocol comprises (1) lysis of a single cell in a tube without purification, (2) first-strand cDNA synthesis with the first primer tailed w
45、ith oligo dT, (3) elimination of the unreacted first primer, (4) poly (dA) tailing of the cDNA, (5) second-strand cDNA synthesis with the second primer tailed with oligo dT, and (6) 20-cycle, directional PCR with the two primers. To prepare the template for the isothermal linear amplification with T
46、7 RNA polymerase to synthesize labeled cRNAs for microarray hybridization, the promoter sequence is added to the cDNA with another round of PCR. The promoter-tagged cDNA is purified with gel electrophoresis and amplified with one final cycle of PCR.,A global single-cell cDNA amplification method for
47、 quantitative microarray analysis.,Abstract Heat shock protein 90 is a new target of antitumor drug, the inhibitor of Hsp90 fight against tumor by destroy and degrade the structure of protein. In recent years, looking for Hsp90 inhibitor is not only via structure modifying of natural products, but a
48、lso via high throughput screening and computer aided drug design to find and synthesize new kinds of Hsp90 inhibitor. Anyway, Hsp90 inhibitor has considered as an important biology target and to pay more and more attention. This review describes recent developments of small molecule Hsp90 inhibitors
49、.,Progress in the study of heat shock protein 90 inhibitors,Polyketides are important bioactive natural products biosynthesized by bacteria, fungi, and plants. The enzymes that synthesize polyketides are collectively referred to as polyketide synthases (PKSs). Because many of the natural hosts that produce polyketides are difficult to culture or manipulate, establishing a universal heterologous host that is genetically tractable has become an important goal toward the engineered biosynthesis of polyketides and analogues. Here, we summariz
