1、 Excitatory effect of oxytocin on uterine smooth muscle Shi-Hong Zhang（张世红）Dept of PExperimental pharmacology of reproductive system Pituitrin(垂体后叶素垂体后叶素)Oxytocin(催产素催产素)Ergot alkaloids(麦角生物碱麦角生物碱)Prostaglandins(PGs,前列腺素前列腺素)Pituitrin(垂体后叶素垂体后叶素)oxytocin vasopressin 垂体垂体Oxytocin(OXT,催产素催产素)1.Pharmac
2、ological effects:(1)Uterus:Elevating both the frequency and force of uterine contraction,at rhythm similar to natural labor,highly dependent on estrogen level and condition of uterus.(2)Breast:milk ejection.(3)Blood vessel:vasodilation at large dose.2.Clinical uses:-to expedite delivery and induced
3、labor;-to reduce the incidence and severity of postpartum hemorrhage.Oxytocin(OXT,催产素催产素)3.Adverse effects:-Intrauterine asphyxia or uterine rupture(overdose)-Allergy4.Contraindications:-hypertonic dysfunction of uterus -abnormality in birth canal -abnormal fetal positionOxytocin(OXT,催产素催产素)Ergot al
4、kaloids(麦角生物碱麦角生物碱)Ergometrine(麦角新碱麦角新碱),Ergotamine(麦角胺麦角胺),Ergotoxine(麦角毒麦角毒)1.Pharmacological effects:(1)Uterus(ergometrine):-strongly stimulating uterine smooth muscle,both the body and the cervix;ready to induce tonic contraction at larger dose.(2)Blood vessel(ergotamine):-contracting peripheral
5、 blood vessels and cerebral vessels.(3)blocking receptor(ergotoxine):2.Clinical uses:(1)Endometrorrhagia(子宫出血子宫出血),and puerperal replacement of uterus(产后子宫复位产后子宫复位):ergometrine;(2)Migraine(偏头痛偏头痛):ergotamine;(3)Hibernation therapy(冬眠疗法冬眠疗法):dihydroergotoxine+pethidine+promethazine.Ergot alkaloids(麦角
6、生物碱麦角生物碱)3.Adverse effects:-nausea,vomiting,BP ;-allergic reaction;-prohibited in expediting delivery and induced labor.Ergot alkaloids(麦角生物碱麦角生物碱)Prostaglandins(PGs,前列腺素前列腺素)Dinoprostone(地诺前列酮地诺前列酮,PGE2)Dinoprost(地诺前列素地诺前列素,PGF2)1.Pharmacological effect:stimulating uterine smooth muscle.2.Clinical
7、uses:to induce labor2.Adverse effects:GI reaction,attack of asthma(PGF2);glaucoma(PGE2).Objectives To observe the excitatory effect of oxytocin on uterine smooth muscles and its dose dependent manner.Methods1.Estrodiol pretreatment(0.2mg)on rats(200 g 40g)24hr before experiment;2.Prepare uterus smoo
8、th muscle specimen.MethodsUterus of the ratMethodsUterus of the ratMethodsPerfusion systemMethods Set parameters of recording system:Input pattern:tensility Gain:200-500 Lower frequency:direct current Upper frequency:10 Hz Sampling interval:2 ms Preload:1gMethods1.The specimen is stabilized in the p
9、erfusion chamber for 15-30 min until the contractive force and rhythm become regular.Record the basal contractive force and frequency(optional).2.Add 0.02 ml,0.04 ml,0.14 ml oxytocin(0.01U/ml)into the perfusion system.Record the maximal force and frequency(optional)of contraction at each drug concen
10、tration accordingly.Methods3.Add 0.04 ml,0.14 ml oxytocin(0.1U/ml)into the perfusion system.Record the maximal force and frequency(optional)of contraction at each drug concentration accordingly.4.Add 0.04 ml,0.14 ml oxytocin(1U/ml)into the perfusion system.Record the maximal force and frequency(opti
11、onal)of contraction at each drug concentration accordingly.Methods5.Add 0.04 ml,0.14 ml oxytocin(10U/ml)into the perfusion system.Record the maximal force and frequency(optional)of contraction at each drug concentration accordingly.MethodsNotice:1Avoid injury and excessive stretch of uterus during t
12、he whole process of preparation.2Control the gas bubbles in a proper rate in case of impact on uterus contraction.3Record a period of baseline of contraction before drug treatment in order to compare that before and after.4Add drug in the order as prescribed.Lab report1题目2署名:作者和合作者姓名及单位。3结构式摘要（目的，方法，结果，结论）。4材料和方法:主要实验材料，标本制备方法，仪器装置连接和仪器参数，实验数据记录条件，观察指标。5结果:实验结果的文字性描述，作量效曲线。6讨论:回答思考题。Learning issue1缩宫素对子宫的作用及特点。2为什么实验前用雌二醇处理动物？3.缩宫素与麦角新碱对子宫平滑肌的作用特点有何不同？
