1、1.Immune response Simple process of HI:1.Common rule of Ab productionSecondary Immune ResponseWhen the immunized individual after a primary contact with antigen is injected by a second injection of the same antigen,production of antibody becomes apparent,this response is called the secondary respons
2、e.Primary IR Secondary IRAntigen The first contact of an A second injection of the individual with an Ag same Aglatent period long(1W，2-3W)short(1-3day)slow of Ab productionAb Conc.low in serum high in serum Maintain Time short,decrease quickly longertype of Ig IgM IgGAffinity lower higherpresent of
3、 Ag M,DC B cell IgIgMIgG10day3dayLatent periodendosomeTransportation combinationdegradationexpressionendocytosisAPCCD4MHCIItranscriptionDNAmRNAVirus（endogenous Ag）translationPrdegradationCD8CTLMHCITarget cellTCRICAM-1LFA-1 APC(CMs)MHC-IIAgB7/BB1VLA-4VCAM-1CD2CD58Cell activation,（CK）cell differentiat
4、ion and clone proliferation TH(CMRs)TCRCD3CD4CD28CTLA4Signal 1Signal 2IL-2IL-2RMHC-AgAPCTCR/CD3B7/BB1CD28THSignal 1Signal 2 IL-2 production clone proliferationAPCTHSignal 1Signal 2 not IL-2 production clone anergyinactivationAnti-B7AbCTLA4 IgAnti-CD28Interdiction of co-stimulatory signalsTHAPCMHCTCR
5、AgCD28B7B7Transfection B7geneCTLMHCTCRAgCD28Tumor cellsignal1signal2CTLMHCTCRAgCD28Tumor cellsignal1signal2CTL activatedKiller tumor cellCTL activatedKiller tumor cellSignal 2：CD3singalB cell activationB cellAgSIg12Signal 1T cell B7CD28ICAM-1LFA-1CD3TCRCD4MHC-IICD40LCD40LFA-1ICAM-1Th activationCKAg
6、processpresentCKRAgBmBBBBPCIgMIgGIgAIgEBmBmBmBmActivate ThActivate BIL-2，4，5，6IL-1processpresentprocesspresentproliferationingestioningestionMHC-IIMHC-IITCRTCRM M differentiationc.Interaction between M and B cellssignalsignalB cell B cell I type:TIAg is polyclonal stimulator II type:multiple repeat-
7、lined antigenic determinant makes receptor linkageTI-Ag response3.3.Immune Memory-Secondary response*Immune memory cells:THm Bm *Involved in Humoral immunity and cell-mediated immunty.*Ab conc.increases，class switching of Ab，and affinity maturation of Ab4.Effect of Humoral ImmunityOpsonization of Ab
8、BacteriaAnti-bacteria Ab M FcrRBoost up lick up actionTarget cell M ,NKDissolution of target cell IgGSurface AgFcrRAntibody-dependent cell-mediated cytotoxicity,ADCC I.IntroductionTD-AgImmune systemsecondaryTDTHTcCKCytotocixity2.Major Functions of CMIII.CD4 T-mediated immunityActivation of CD4 T cel
9、lsIL-2RIL-2R APC(CMs)TH(CMRs)CD28CTLA4CD3TCRCD4ICAM-1LFA-1MHC-IIAgB7/BB1VLA-4VCAM-1CD2CD58Signal1 Signal 2IL-2IL-2RCell activation,（CK）cell differentiation and clone proliferationAPCTAPCTAPCTTTTTIL-2IFNrTNFTM restM activationCK of DTH reactionIII.CD8 T-mediated immunityActivation of CD8 T cellsICAM-1LFA-1 Target cell(CMs)MHC-IAgCD2CD58 Tc(CMRs)TCRCD3B7/BB1CTLA4Signal 1Signal 2IL-2RCD8Cell activation,differentiation,clone proliferation and secreted effect molecules.IL-2TH1Effector TcRest TcTarget cellAPCMHCIMHCIIIL-2，IL-6，IFNTCRdeathTarget cell
