1、Initiation of transcription20.1 Introduction20.1 Introduction20.2 Eukaryotic RNA polymerases consist of many subunits 20.2 Eukaryotic RNA polymerases consist of many subunits 20.3 Promoter elements are defined by mutations and footprinting 20.3 Promoter elements are defined by mutations and footprin
2、ting 20.4 RNA polymerase I has a bipartite promoter 20.5 RNA polymerase III uses both downstream and upstream promoters 20.5 RNA polymerase III uses both downstream and upstream promoters 20.5 RNA polymerase III uses both downstream and upstream promoters 20.5 RNA polymerase III uses both downstream
3、 and upstream promoters 20.6 The startpoint for RNA polymerase II 20.7 TBP is a universal factor20.7 TBP is a universal factor20.7 TBP is a universal factor20.8 The basal apparatus assembles at the promoter20.8 The basal apparatus assembles at the promoter20.8 The basal apparatus assembles at the pr
4、omoter20.9 A connection between transcription and repair 20.9 A connection between transcription and repair 20.9 A connection between transcription and repair 20.9 A connection between transcription and repair 20.10 Promoters for RNA polymerase II have short sequence elements 20.10 Promoters for RNA
5、 polymerase II have short sequence elements 20.10 Promoters for RNA polymerase II have short sequence elements 20.10 Promoters for RNA polymerase II have short sequence elements ModuleConsnesusDNA boundFactorTATA boxTATAAAA10bpTBPCAAT boxGGCCAATCT22bpCTF/NF1GC boxGGGCGG20bpSP1OctamerATTTGCAT20bpOct-
6、1OctamerATTTGCAT23bpOct-2kBGGGACTTTCC10bpNF kBATFGTGACGT20bpATF20.10 Promoters for RNA polymerase II have short sequence elements ModuleConsnesusDNA boundFactorTATA boxTATAAAA10bpTBPCAAT boxGGCCAATCT22bpCTF/NF1GC boxGGGCGG20bpSP1OctamerATTTGCAT20bpOct-1OctamerATTTGCAT23bpOct-2kBGGGACTTTCC10bpNF kBAT
7、FGTGACGT20bpATF20.10 Promoters for RNA polymerase II have short sequence elements 20.11 Enhancers contain bidirectional elements that assist initiation 20.11 Enhancers contain bidirectional elements that assist initiation 20.11 Enhancers contain bidirectional elements that assist initiation 20.11 En
8、hancers contain bidirectional elements that assist initiation 20.11 Enhancers contain bidirectional elements that assist initiation 20.11 Enhancers contain bidirectional elements that assist initiation 20.12 Independent domains bind DNA and activate transcription 20.12 Independent domains bind DNA a
9、nd activate transcription 20.12 Independent domains bind DNA and activate transcription 20.12 Independent domains bind DNA and activate transcription 20.12 Independent domains bind DNA and activate transcription 20.13 Interaction of upstream factors with the basal apparatus 20.13 Interaction of upstream factors with the basal apparatus 20.13 Interaction of upstream factors with the basal apparatus 20.13 Interaction of upstream factors with the basal apparatus 20.13 Interaction of upstream factors with the basal apparatus Summary
