1、续前续前min%11maxmax，shcmE续前例：例：45. 315. 388. 170. 155036127836155036127812AAAAVB，三处最大吸收，定性鉴别：药典规定续前续前%06. 01002101 . 1%/101 . 1100/101 . 1143505. 0334%11肾上腺酮mlmgmlglEACcmlECA定量依据：续前要求单色光前提：iECAmLgCigW 求含样过程：已知稀释)/(/)(lEAmLgCi100/lALmolCi/或%100%样CCii练习mLgmLglEAC/0 .20100/00200. 01207414. 0练习mLgmLgCi/104
2、5. 2100/1045. 21207507. 053mLgC/1050. 2100101001050. 252样%0 .98%1001050. 21045. 2%100%5512样CCBi练习%)0 .764(591. 01001025000.20367255iEAmLmLmLmgmlmLHCL求已知测移取稀至样品稀至吡啶 mLgmLglEACi/1092. 7100/1092. 7764591. 064%0 .99%10025010100100 . 21092. 7%100%26样CCii条件前提：固定仪器和测定CACKA样样同上条件固定条件查得测定样品曲线分别测定配制标准系列过程：CAA
3、CA标样标样标样标样AACCAACC示例mLmgmLmLmLmg250 . 32550/200. 0样品标样分别移取续前标样与样品浓度相近；截距为固定仪器和测定条件；前提：0标样和分别测定一定过程：AASiSiAACCsCiCCSiSiAAmm%100%mmii练习mLgCCii/1 .98518. 0508. 01000100000.25105 . 2%1 .98%100%12标示量标示量iCB练习1207508. 01050. 2iiClEACmLgCi/1 .98%1 .98%100%12标示量标示量iCB续前cbaAAAA总定量依据：两组分在各自两组分在各自max下不重叠下不重叠分别按
4、单组分定量分别按单组分定量aaaaaaEACCEA1111由bbbbbbEACCEA2222由bbaaAEAE222111；测定；测定过程：续前1测测A1b组分不干扰组分不干扰可按单组分定量测可按单组分定量测Ca 2测测A2a组分干扰组分干扰不能按单组分定量测不能按单组分定量测Ca babaaaAEEAE2222111；和测定；测定过程：aaaaaaEACCEA1111由bbaababaCECEAAA22222由baababECEAC222续前（1）解线性方程组法）解线性方程组法（2）等吸收双波长消去法）等吸收双波长消去法（3）系数倍率法）系数倍率法babababaAEEAEE22221111
5、；和测定；和测定bbaababaCECEAAA11111bbaababaCECEAAA22222bababbabbaaEEEEEAEAC12211221babaabaababEEEEEAEAC12212112babababaAAAAAA222111babbaabababaAAAAAAAAA)()(212121aaAAa2121和的等吸收点选bbbbbbbbaCECEEAAA)(2121bbabbbabEAEEAC21续前bbAAb 2 1 2 1和的等吸收点选aaaaaaabaCECEEAAA)(21 2 1abaaabaaEAEEAC 2 1前提：干扰组分前提：干扰组分b不成峰形不成峰形 无
6、等吸收点无等吸收点续前 倍率因子令KAAbb21)()()()(1212112212aabbababbababaAKAAKAAAAAKAKAAbA1bA2aaaaabaCEKEAKAA)(1212的干扰消除了bCAaba练习9 .927%11cmEmLgmLgCi/10900. 4100/10900. 419 .927463. 064mLgC/10000. 55052001000.1063样%0 .98%10010000. 510900. 4%100%66样咖啡酸CCi5052001稀释因子注：练习%11cmE12000 .100120001010%11MEcmmLgmLglETlEACi/10165. 3100/10166. 311200417. 0lglg64mLgC/10000. 410022500500. 06样%15.79%10010000. 410165. 3%100%66样样品CCi
