12345678pSC1019pSC1011011121314151617181920212223PCR原理图 333变性、退火延伸变性、退火、延伸32425262728 2930313233polylinker3435 RBSPO 363738394041424344 4546474849 50 515555 52IBamHI535455565750-100mmol/LCaCl2 58596061626364pBR322AmpTet65666768滤膜(固定抗体)+69菌落沉淀素70ACTGAAGGCT71AmPori7273741 1目的基因在目的基因在757677P7879mRNASD80 818283848586