1、Technology技术技术Characters特特征征Applications 功功能性能性PCR产产物测序物测序Denovo 新新测序测序Re-seq 重重测序测序GT1000/100WGSCBC1st Gen第第一代一代1.1Gel 平平板胶板胶NA1.2CE 毛毛细管细管-四色荧光四色荧光NA2nd Gen新一新一代代2.1微乳液微乳液PCRLRNANANANASRNANANANA2.2高通量高通量/无反应仓无反应仓NANANANA2.3单分子单分子NANA1000Future下一下一代代3.1化学化学/纳米技术纳米技术NANA10003.2纳米技术纳米技术NANA1003.3纳米技术
2、纳米技术NANA100Key TechnologyKey Technology关键技术关键技术1 1st st Gen Gen 第第一代一代2 2ndnd Gen Gen 第第二代二代3 3rdrd Gen Gen 第第三代三代4 4thth Gen Gen 第第四代四代SangerSangerCyclic Cyclic ArrayArraySMSSMSDirectDirectHybridizationHybridization分子杂交分子杂交Enzyme Enzyme 酶酶介导生化反应介导生化反应Fluor-LabelFluor-Label荧荧光标记底物光标记底物PCRPCR扩增扩增Elec
3、trophoresisElectrophoresis电泳电泳Micro/NanoMicro/Nano微微纳加工纳加工Fluor-DetectionFluor-Detection荧荧光信号识别光信号识别SMDSMD单单分子信号检测分子信号检测Electro-SignalElectro-Signal电电信号信号识别识别Micro-FluidicsMicro-Fluidics微微量液流控制量液流控制Sequencing Method Based onReal-time Pyrophosphate（焦磷酸焦磷酸）Ronaghi M,Uhlen M,Nyren PADepartment of Bioch
4、emistry and Biotechnology,The Royal Institute of Technology,Stockholm,Sweden Science 1998;281:363,365将将PCR反应物包被于反应物包被于“油包水油包水”的乳化剂中，的乳化剂中，扩增过程就可扩增过程就可以在每一滴乳化剂内独立进行以在每一滴乳化剂内独立进行 Each chip contains thousands of ZMWs.Each ZMW is a cylindrical hole tens of nanometers in diameter,perforating a thin metal(e.g.)film supported by a transparent substrate 340,00070,000Roche 454J.Watson932507.4x2341,000SOLiD J.Lupski2383530 x375SolexaAn African Male3,6813540 x40250An Han Chinese2,9503536x354001st Korean Male1,64735,7429x152502nd Korean Male1,91036,88,10628x30200HelicosS.Quake2,7253228x448
