1、2022-12-261色谱法的理论与实践2022-12-262一、色谱基础理论概述一、色谱基础理论概述2022-12-2632022-12-264 二、色谱法的分类二、色谱法的分类 2022-12-265按色谱过程的分离机制分类 2022-12-2662022-12-2672022-12-268填充柱毛细管柱毛细管电色谱毛细管电泳柱色谱平面色谱逆流分配色谱GC LC SFCCEC 色谱法经典液相色谱HPLC纸色谱法薄层色谱法薄膜色谱法2022-12-269 2022-12-26102022-12-2611掌握2022-12-2612W0.05h=x=h/20峰的对称性hA Bd1xWW0.05
2、h0.05h2022-12-2613峰面积(A)峰高(h)tR tR掌握2022-12-2614Nmv基线漂移掌握2022-12-26152022-12-26162022-12-26172022-12-2618tR1tR3tR2t0tR3tR2tR1mvtime进样2022-12-26192022-12-26202022-12-2621熟悉2022-12-2622h0.607h x2022-12-2623h0.5hW1/22022-12-2624 W2022-12-2625 Wh0.5h0.607hW1/22022-12-26262022-12-2627n分离参数n分离参数用于衡量分离条件的优劣，常用分离度，表示。u分离度（resolution，R）uR=u即相邻两峰分开的距离是平均峰宽(W1+W2)/2的几倍。R大,分离效果好。2（tR2-tR1）W1+W22022-12-26282022-12-26292022-12-26302022-12-26312022-12-26322022-12-2633
