1、1Outline&穴位肥大细胞在针刺镇痛效应中的作用&穴位胶原纤维在针刺镇痛效应中的作用&穴位注射组胺的镇痛效应&手针和电针穴位信号的启动机制THE JOURNAL OF SCIENCE&HEALING,2008THE JOURNAL OF SCIENCE&HEALING,2008，4 4（3 3），），170-177170-177Animal modelAnimal model ：AA AA (acute arthritis)(acute arthritis)RatRatComplete Freunds adjuvant Acupoint selectedAcupoint selected：
2、Zushanli Zushanli (ST36ST36)Treat effectTreat effect：Ease painEase pain，Pain Pain thresholdthresholdIDGroup Name组别组别Average SEM(%)AControl正常对照组正常对照组1.90.1B1Acupuncture at ST36穴位针刺组穴位针刺组11.60.6*B2Acupuncture at Nearby-ST36旁开针刺组旁开针刺组8.90.3*C1Injection of DSCG at ST36色甘酸钠注射组色甘酸钠注射组1.70.1*C2Injection of
3、 NaCl at ST36生理盐水注射组生理盐水注射组1.40.1*D1Acupuncture after DSCG at ST36色甘酸钠注射色甘酸钠注射针刺组针刺组6.20.4*D2Acupuncture after NaCl at ST36生理盐水注射生理盐水注射针刺组针刺组8.90.4*D3Acupuncture opposite side after DSCG色甘酸钠注射色甘酸钠注射对侧针刺组对侧针刺组9.30.1*各组别大鼠痛阈变化率峰值比较（各组别大鼠痛阈变化率峰值比较（2020分钟时）分钟时）(n=6)(n=6)Note:*P0.05 vs.Group A;P0.01,P0.0
4、1,P0.05 vs.Group B1;P0.05 vs.Group B1;P0.01,P0.01,P0.05 vs.Group D1P0.05 vs.Group D1Group Name组别组别MC Degranulation Ratio(Average SEM)()SkinMuscleControl正常对照组正常对照组29.21.226.31.8Acupuncture穴位针刺组穴位针刺组48.93.9*32.23.1*Acupuncture after DSCG色甘酸钠注射针刺组色甘酸钠注射针刺组33.72.828.52.3Acupuncture after NaCl生理盐水注射针刺组生理
5、盐水注射针刺组50.42.5*29.72.3穴区组织肥大细胞脱颗粒率统计穴区组织肥大细胞脱颗粒率统计 (n=6)(n=6)Note:*P0.01,*P0.05 vs.Control Group;P0.01 vs.Acupuncture Group.P0.01 vs.Acupuncture Group.Degranulationof cellsTHE JOURNAL OF SCIENCE&HEALING,2008THE JOURNAL OF SCIENCE&HEALING,2008，4 4（3 3），），170-177170-177lThe acupuncture effect is posit
6、ive relationship to the degranulation of mast cell at acupoint.lWhat is key factor conduce the degranulation of mast cell during acupuncture manipulation?lOur focus is on the collagen at acupoint.2023-1-2212To observe the function of collagen fibers in MA analgesia.7 Animal groups setting:normal gro
7、up(N):Normal healthy animal control group(M):Animal with AA injection of collagenase at Zusanli(ST36)group(C)manual acupuncture group with rotation manipulation(R)or lift-thrust manipulation(L),rotation group with collagenase pretreatment to destroy Zusanli(ST36)collagen fibers(CR),lift-thrust group
8、 with collagenase pretreatment to destroy Zusanli(ST36)collagen fibers(CL),lift-thrust group pretreated with collagenase to destroy Dubi(ST35)collagen fibers(CDL)(R)(L)2023-1-2213Analgesia effects observed in different groupsGroupnPain Threshold(PT)4d Before MA(s)After MA(s)Change Rate(%)MC Degranul
9、ation Ratio(%)N89.190.65-10.62.6M85.610.48*-14.15.9C85.900.24*5.590.52-5.111.94.7R85.440.37*7.520.6538.575.74.4L86.130.82*8.501.5639.176.95.9CR85.270.62*5.390.573.310.42.4CL86.050.43*6.260.793.812.42.3注：较正常组痛阈：注：较正常组痛阈：*P0.01P0.01；较处理前痛阈：；较处理前痛阈：P0.05P0.05；较捻转组；较捻转组(R)(R)痛阈变化率：痛阈变化率：P0.05P0.05；较捻转组较
10、捻转组(L)(L)痛阈变化率：痛阈变化率：P0.05P0.05；较较正常组或模型组正常组或模型组:P0.05 P0.05；较捻转组或提插组；较捻转组或提插组:P0.05 P0.05。2023-1-2214Analgesia effects observed in different groupsGroupnPain Threshold(PT)4d Before MA(s)After MA(s)Change Rate(%)MC Degranulation Ratio(%)N89.190.65-10.62.6M85.610.48*-14.15.9C85.900.24*5.590.52-5.111.
11、94.7R85.440.37*7.520.6538.575.74.4L86.130.82*8.501.5639.176.95.9CR85.270.62*5.390.573.310.42.4CL86.050.43*6.260.793.812.42.3注：较正常组痛阈：注：较正常组痛阈：*P0.01P0.01；较处理前痛阈：；较处理前痛阈：P0.05P0.05；较捻转组；较捻转组(R)(R)痛阈变化率：痛阈变化率：P0.05P0.05；较捻转组较捻转组(L)(L)痛阈变化率：痛阈变化率：P0.05P0.05；较较正常组或模型组正常组或模型组:P0.05 P0.05；较捻转组或提插组；较捻转组或提插
12、组:P0.05 P0.05。01 与组胺穴位注射组比较，P0.Note:*P0.01 与手针针刺组比较（vs.通过H1受体进一步传递针刺信号01 与手针针刺组比较（vs.较捻转组(R)痛阈变化率：P0.Pain Threshold(PT)组胺H1拮抗剂穴位注射组注：与 正常组相比较：*P0.01 与生理盐水穴位注射组比较；MC Degranulation Ratio(%)After SDCG StabilizedAfter MA(s)Pain threshold of rat when shield mast cell degranulationInjection drugs for prev
13、ent degranulation of MC2023-1-2215Analgesia effects observed in different groupsGroupnPain Threshold(PT)4d Before MA(s)After MA(s)Change Rate(%)MC Degranulation Ratio(%)N89.190.65-10.62.6M85.610.48*-14.15.9C85.900.24*5.590.52-5.111.94.7R85.440.37*7.520.6538.575.74.4L86.130.82*8.501.5639.176.95.9CR85
14、.270.62*5.390.573.310.42.4CL86.050.43*6.260.793.812.42.3注：较正常组痛阈：注：较正常组痛阈：*P0.01P0.01；较处理前痛阈：；较处理前痛阈：P0.05P0.05；较捻转组；较捻转组(R)(R)痛阈变化率：痛阈变化率：P0.05P0.05；较捻转组较捻转组(L)(L)痛阈变化率：痛阈变化率：P0.05P0.05；较较正常组或模型组正常组或模型组:P0.05 P0.05；较捻转组或提插组；较捻转组或提插组:P0.05 P0.05。collagen fibers of skin and muscleMast cells in skinGr
15、oupNormalGroupInjectControlcollagen fibers of skin and muscleMast cells in skinConnective Tissue Research,2008Connective Tissue Research,2008，5050（2 2）:110-120:110-120Acupunc.whirling Acupunc.lift and thrust collagen fibers of skin and muscleMast cells in skincollagen fibers of skin and muscleMast c
16、ells in skinConnective Tissue Research,2008Connective Tissue Research,2008，5050（2 2）:110-120:110-120 Mast cells degranulation before and after collagen destroyed Collagen destroyedCollagen destroyedConnective Tissue Research,2008Connective Tissue Research,2008，5050（2 2）:110-120:110-120collagen fiber
17、s and mast cells in normal acupointcollagen fibers enlacement and mast cells degranulation while whirlingcollagen fibers tightened and mast cells degranulated while lifting and thrustingcollagen fibers were destroyed and mast cells had no degranulationConnective Tissue Research,2008Connective Tissue
18、 Research,2008，5050（2 2）:110-120:110-120较捻转组或提插组:P0.注：较正常组痛阈：*P0.较正常组或模型组:P0.75.degranulated while lifting and thrustingAnimal model：AA (acute arthritis)RatDubi(ST35)collagen fibers(CDL)与 模型组相比较：P0.穴位注射组胺的镇痛效应The relationship betweenmast cell function and acupuncture effectPain Threshold(PT)The degr
19、anulation of mast cells较处理前痛阈：P0.What is the key factor to degranulationConnective Tissue Research,2008，50（2）:110-1202023-1-2220The mast cells in acupoint may translate the mechanical signal into the biological information of neural system.The collagen and mast cells degranulation are very important
20、 to the manual acupuncture analgesia effect.Is this the same as electric acupuncture?2023-1-2221组别组别n 痛阈痛阈(s)痛阈变化率痛阈变化率(%)肥大细胞脱颗肥大细胞脱颗粒率粒率(%)正常组正常组8 9.190.65-10.6模型组模型组8 5.610.48*-14.1手针组手针组8 7.520.6538.5 75.4电针组电针组8 8.671.0645.9 73.3胶原酶胶原酶+手针组手针组8 5.390.573.2 10.4胶原酶胶原酶+电针组电针组8 8.301.2347.6 19.0色甘酸
21、钠色甘酸钠+手针组手针组8 6.000.485.8 25.1色甘酸钠色甘酸钠+电针组电针组8 7.340.7027.5 29.5手针及电针治疗前后手针及电针治疗前后AAAA大鼠痛阈和痛阈改变率以及穴大鼠痛阈和痛阈改变率以及穴区肥大细胞脱颗粒率的变化区肥大细胞脱颗粒率的变化注：与注：与 正常组相比较：正常组相比较：*P0.05P0.05；与；与 模型组相比较：模型组相比较：P0.05P0.05；与手针组与手针组相比较：相比较：P0.05 P0.05；P0.01 P0.01；与电针组相比较：；与电针组相比较：P0.05P0.05。组别组别n 痛阈痛阈(s)基础痛阈基础痛阈造模后痛阈造模后痛阈治疗后
22、痛阈治疗后痛阈空白对照组空白对照组129.040.209.380.199.270.17模型组模型组128.900.406.480.286.580.35生理盐水对照组生理盐水对照组129.200.286.090.306.610.31手针组手针组129.520.186.580.178.770.26组胺注射组组胺注射组129.210.206.270.228.500.28各组痛阈测量比较各组痛阈测量比较组别组别n 痛阈痛阈(s)基础痛阈基础痛阈造模后痛阈造模后痛阈治疗后痛阈治疗后痛阈空白对照组空白对照组129.040.209.380.199.270.17模型组模型组128.900.406.480.28
23、6.580.35生理盐水对照组生理盐水对照组129.200.286.090.306.610.31手针组手针组129.520.186.580.178.770.26组胺注射组组胺注射组129.210.206.270.228.500.28各组痛阈测量比较各组痛阈测量比较针刺研究，针刺研究，20102010，3535（2 2）：）：99-10399-103组别组别n 痛阈痛阈(s)基础痛阈基础痛阈造模后痛阈造模后痛阈治疗后痛阈治疗后痛阈空白对照组空白对照组129.040.209.380.199.270.17模型组模型组128.900.406.480.286.580.35生理盐水对照组生理盐水对照组12
24、9.200.286.090.306.610.31手针组手针组129.520.186.580.178.770.26组胺注射组组胺注射组129.210.206.270.228.500.28各组痛阈测量比较各组痛阈测量比较针刺研究，针刺研究，20102010，3535（2 2）：）：99-10399-103lift-thrust group pretreated with collagenase to destroy针刺研究，2010，35（2）：99-103色甘酸钠预处理胺注射组Zusanli(ST36)collagen fibers(CR),01 与空白组比较（vs.Zusanli(ST36)c
25、ollagen fibers(CL),Acupuncture穴位皮下注射组胺的镇痛疗效各组别大鼠痛阈变化率峰值比较（20分钟时）(n=6)collagen fibers and mast cellsBefore Acupuncture色甘酸钠预处理胺注射组较处理前痛阈：P0.Results from Experiment穴位肥大细胞在针刺镇痛效应中的作用各组足三里穴位处脱颗粒比较各组足三里穴位处脱颗粒比较组别组别N脱颗粒率脱颗粒率空白组空白组121233.5933.590.72模型组模型组121239.7139.712.09生理盐水穴位注射组生理盐水穴位注射组121236.6736.672.3
26、6手针针刺组手针针刺组121257.6157.611.42组胺穴位注射组组胺穴位注射组121257.0357.032.95色甘酸钠预处理胺注射组色甘酸钠预处理胺注射组131325.4025.401.80色甘酸钠预处理针刺组色甘酸钠预处理针刺组121236.0336.032.28组胺组胺H1拮抗剂预处理针刺组拮抗剂预处理针刺组121251.5451.542.32组胺组胺H1拮抗剂组胺注射组拮抗剂组胺注射组121237.1337.131.90组胺组胺H1拮抗剂穴位注射组拮抗剂穴位注射组222232.2432.241.40P0.01 P0.01 与空白组比较（与空白组比较（vs.controlvs.
27、control）；）；P0.01 P0.01 与模型组比较（与模型组比较（vs.modelvs.model）；）；P0.01 P0.01 与与手针针刺组比较（手针针刺组比较（vs.controlvs.control）；）；P0.01 P0.01 与生理盐水穴位注射组比较；与生理盐水穴位注射组比较；P0.01 P0.01 与组胺穴位与组胺穴位注射组比较，注射组比较，P0.01 P0.01 与组胺与组胺H1H1拮抗剂穴位注射组比较。拮抗剂穴位注射组比较。针刺研究，针刺研究，20102010，3535（2 2）：）：99-10399-103各组足三里穴位处脱颗粒比较各组足三里穴位处脱颗粒比较组别组别
28、N脱颗粒率脱颗粒率空白组空白组121233.5933.590.72模型组模型组121239.7139.712.09生理盐水穴位注射组生理盐水穴位注射组121236.6736.672.36手针针刺组手针针刺组121257.6157.611.42组胺穴位注射组组胺穴位注射组121257.0357.032.95色甘酸钠预处理胺注射组色甘酸钠预处理胺注射组131325.4025.401.80色甘酸钠预处理针刺组色甘酸钠预处理针刺组121236.0336.032.28组胺组胺H1拮抗剂预处理针刺组拮抗剂预处理针刺组121251.5451.542.32组胺组胺H1拮抗剂组胺注射组拮抗剂组胺注射组12123
29、7.1337.131.90组胺组胺H1拮抗剂穴位注射组拮抗剂穴位注射组222232.2432.241.40P0.01 P0.01 与空白组比较（与空白组比较（vs.controlvs.control）；）；P0.01 P0.01 与模型组比较（与模型组比较（vs.modelvs.model）；）；P0.01 P0.01 与与手针针刺组比较（手针针刺组比较（vs.controlvs.control）；）；P0.01 P0.01 与生理盐水穴位注射组比较；与生理盐水穴位注射组比较；P0.01 P0.01 与组胺穴位与组胺穴位注射组比较，注射组比较，P0.01 P0.01 与组胺与组胺H1H1拮抗剂
30、穴位注射组比较。拮抗剂穴位注射组比较。针刺研究，针刺研究，20102010，3535（2 2）：）：99-10399-103l组胺参与到了针刺麻醉的穴位局部机制组胺参与到了针刺麻醉的穴位局部机制l通过通过H1受体进一步传递针刺信号受体进一步传递针刺信号研究阐明了手针和电针穴位针刺有效信号的启动机制研究阐明了手针和电针穴位针刺有效信号的启动机制MAEAElectric A.10382.0000010384.00000seconds-0.199992-0.0999960.0000000.099996Volts 心电图91.20613493.14669095.08724697.027802mmHg动
31、脉血压0.00000036.75320173.506401110.259602mmHg心室内压-41.509399-21.509399-1.50939918.49060138.490601Volts正中神经放电-0.019582-0.0093089.7E-0040.011241Volts交感神经放电10580.00000seconds-0.447150-0.247150-0.0471500.1528500.352850Volts 心电图95.61133897.52356599.435792101.348018mmHg动脉血压0.00000036.59941373.198826109.79824
32、0mmHg心室内压-13.244812-7.244812-1.2448124.75518810.755188Volts正中神经放电-0.069308-0.039308-0.0093080.0206920.050692Volts交感神经放电30Degranulationof cellsTHE JOURNAL OF SCIENCE&HEALING,2008THE JOURNAL OF SCIENCE&HEALING,2008，4 4（3 3），），170-177170-17731&The acupuncture effect is positive relationship to the degr
33、anulation of mast cell at acupoint.&What is key factor conduce the degranulation of mast cell during acupuncture manipulation?&Our focus is on the collagen at acupoint.322023-1-2232To observe the function of collagen fibers in MA analgesia.7 Animal groups setting:normal group(N):Normal healthy anima
34、l control group(M):Animal with AA injection of collagenase at Zusanli(ST36)group(C)manual acupuncture group with rotation manipulation(R)or lift-thrust manipulation(L),rotation group with collagenase pretreatment to destroy Zusanli(ST36)collagen fibers(CR),lift-thrust group with collagenase pretreat
35、ment to destroy Zusanli(ST36)collagen fibers(CL),lift-thrust group pretreated with collagenase to destroy Dubi(ST35)collagen fibers(CDL)(R)(L)33Mast cells in skincollagen fibers of skin and muscleor lift-thrust manipulation(L),Injection drugs for prevent degranulation of MCNon-acupointWhat is the ke
36、y factor to degranulationTHE JOURNAL OF SCIENCE&HEALING,2008，4（3），170-177The degranulation of mast cells10.Complete Freunds adjuvantAnalgesia effects observed in different groups73.Change Rate(%)较正常组或模型组:P0.注：较正常组痛阈：*P0.2023-1-2233Analgesia effects observed in different groupsGroupnPain Threshold(PT
37、)4d Before MA(s)After MA(s)Change Rate(%)MC Degranulation Ratio(%)N89.190.65-10.62.6M85.610.48*-14.15.9C85.900.24*5.590.52-5.111.94.7R85.440.37*7.520.6538.575.74.4L86.130.82*8.501.5639.176.95.9CR85.270.62*5.390.573.310.42.4CL86.050.43*6.260.793.812.42.3注：较正常组痛阈：注：较正常组痛阈：*P0.01P0.01；较处理前痛阈：；较处理前痛阈：P0
38、.05P0.05；较捻转组；较捻转组(R)(R)痛阈变化率：痛阈变化率：P0.05P0.05；较捻转组较捻转组(L)(L)痛阈变化率：痛阈变化率：P0.05P0.05；较较正常组或模型组正常组或模型组:P0.05 P0.05；较捻转组或提插组；较捻转组或提插组:P0.05 P0.05。34Acupunc.whirling Acupunc.lift and thrust collagen fibers of skin and muscleMast cells in skincollagen fibers of skin and muscleMast cells in skinConnective
39、 Tissue Research,2008Connective Tissue Research,2008，5050（2 2）:110-120:110-12035Injection drugs for prevent degranulation of MCResults from Experiment针刺研究，2010，35（2）：99-103Electric A.01 与组胺穴位注射组比较，P0.01 与模型组比较（vs.The degranulation of mast cellsZusanli(ST36)collagen fibers(CR),or lift-thrust manipula
40、tion(L),The collagen and mast cells degranulation are very important to the manual acupuncture analgesia effect.01 与手针针刺组比较（vs.Acupuncture after NaCl01 与生理盐水穴位注射组比较；Tail Flick Model较捻转组(R)痛阈变化率：P0.Before Acupuncture10.Mast cells in skinAcupuncture Group.The collagen and mast cells degranulation are
41、very important to the manual acupuncture analgesia effect.Tail Flick Model19.After MA(s)组胺H1拮抗剂组胺注射组Injection drugs for prevent degranulation of MCAfter MA(s)normal group(N):Normal healthy animal色甘酸钠注射对侧针刺组Acupuncture opposite side after DSCGThe relationship betweenmast cell function and acupuncture
42、 effect各组足三里穴位处脱颗粒比较组别组别N脱颗粒率脱颗粒率空白组空白组121233.5933.590.72模型组模型组121239.7139.712.09生理盐水穴位注射组生理盐水穴位注射组121236.6736.672.36手针针刺组手针针刺组121257.6157.611.42组胺穴位注射组组胺穴位注射组121257.0357.032.95色甘酸钠预处理胺注射组色甘酸钠预处理胺注射组131325.4025.401.80色甘酸钠预处理针刺组色甘酸钠预处理针刺组121236.0336.032.28组胺组胺H1拮抗剂预处理针刺组拮抗剂预处理针刺组121251.5451.542.32组胺组
43、胺H1拮抗剂组胺注射组拮抗剂组胺注射组121237.1337.131.90组胺组胺H1拮抗剂穴位注射组拮抗剂穴位注射组222232.2432.241.40P0.01 P0.01 与空白组比较（与空白组比较（vs.controlvs.control）；）；P0.01 P0.01 与模型组比较（与模型组比较（vs.modelvs.model）；）；P0.01 P0.01 与与手针针刺组比较（手针针刺组比较（vs.controlvs.control）；）；P0.01 P0.01 与生理盐水穴位注射组比较；与生理盐水穴位注射组比较；P0.01 P0.01 与组胺穴位与组胺穴位注射组比较，注射组比较，P0.01 P0.01 与组胺与组胺H1H1拮抗剂穴位注射组比较。拮抗剂穴位注射组比较。针刺研究，针刺研究，20102010，3535（2 2）：）：99-10399-103
