尿液分析课件(Urinalysis).ppt

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1、Urinalysis Urinary SystemPurpose General evaluation of health Diagnosis of disease or disorders of the kidneys or urinary tract Diagnosis of other systemic disease that affect kidney function Monitoring of patients with diabetes Screening for drug abuse(eg.Sulfonamide or aminoglycosides)Collection o

2、f urine specimens The first voided morning urine(the most common)Random urine(for emergency)Clean-catch,midstream urine(for urine culture)Attention Need to be examined within 1 hourContents Physical examination Chemical examination Microscopic examinationPhysical examination Appearance Urine volume

3、Specific gravity(SG)Appearance Including color and clarity Color:normally,pale to dark yellow (urochrome)Abnormal color:some drugs cause color changes 1.red urine:causes:hematuria hemoglobinuria myoglobinuria 2.yellow-brown or green-brown urine:bilirubin cause:obstructive jaundiceRed Urine Microscop

4、ic Hematuria Urinary tract source Urethra or bladder Prostate Ureter or kidney Non-Urinary tract source Vagina Anus or rectum Pseudohematuria(non-hematuria related red urine)Myoglobinuria Hemoglobinuria Phenolphthalein Laxatives Phenothiazines Porphyria Rifampin Pyridium Bilirubinuria Phenytoin Pyri

5、dium Red diaper syndrome Foods(Beets,Blackberries,Rhubarb)Red UrineCauses of Asymptomatic Gross Hematuria by Incidence Acute Cystitis(23%)Bladder Cancer(17%)Benign Prostatic Hyperplasia(12%)Nephrolithiasis(10%)Benign essential hematuria(10%)Prostatitis(9%)Renal cancer(6%)Pyelonephritis(4%)Prostate C

6、ancer(3%)Urethral stricture(2%)Appearance Clarity:normally,clear Abnormal color:cloudy urine Causes:1.crystals or nonpathologic salts phosphate,carbonate in alkaline urine (dissolve-add acetic acid)uric acid in acid urine (dissolve-warming to 60)2.various cellular elements:leukocytes,RBCs,epithelial

7、 cellsUrine volume The average adult:1000ml to 2000ml/24h Increase polyuria-more than 2000ml of urine in 24 hours 1.physiological states:water intake,some drugs,intravenous solutions 2.pathologic states:diabetes mellitus,diabetes insipidusUrine volume Decrease Oliguria-less than 400ml of urine in 24

8、 hours Anuria-less than 100ml of urine in 24 hours 1.prerenal:hemorrhage,dehydration,congestive heart failure 2.postrenal:obstruction of the urinary tract (may be stones,carcinoma)3.renal parenchymal disease:acute tubular necrosis,chronic renal failureSpecific gravity(SG)Reflect the density of the u

9、rine Range of 1.001 to 1.040 Increase:Dehydration、Fever、Vomiting Diarrhea Diabetes Mellitus and other causes of Glycosuria、Congestive Heart Failure、Syndrome Inappropriate ADH Secretion(SIADH)、Adrenal Insufficiency failure(urine volume and SG)Decrease:diabetes insipidus (urine volume and SG)Chemical

10、examination Urine PH Protein Glucose Ketones Occult blood Bilirubin Urobilinogen Nitrites Urine PH Normal PH The average is about 6 Range from 59(depends on diet)Higher PH-alkaline urine 1.drugs:sodium bicarbonate 2.classic renal tubular acidosis 3.alkalosis(metabolic or respiratory)Lower PH-acid ur

11、ine 1.drugs:ammonium chloride 2.acidosis(metabolic or respiratory)Protein in urine Reference value Qualitative method:negative Quantitative method:less than 150mg of protein in 24 hours Urine proteins come from plasma protein and Tamm-Horsfall(T-H)glycoprotein Proteinuria-more than 150mg proteins in

12、 urine in 24 hours or qualitative test is positive Proteinuria quantification(depend on the amount of protein)heavy proteinuria-4.0g/24 hours moderate proteinuria-1.04.0g/24 hours minimal proteinuria-1.0g/24 hoursQualitative categories of proteinuria Glomerular proteinuria:1.glomerular diseases dama

13、ge glomerular basement membrane but tubular function is normal 2.selective proteinuria-chiefly albumin nonselective proteinuria 3.heavy proteinuria 4.disease:acute glomerulonephritis Tubular proteinuria 1.Renal tubular disease damage tubular function but glomerular is normal 2.Moderate proteinuria 3

14、.disease:pyelonephritis Overflow proteinuria Excess levels of a protein in the circulation,hemoglobin,myoglobin,etc.The renal function is normal Overflow Causes Hemoglobinuria Myoglobinuria Multiple Myeloma Amyloidosis Proteinuria Causes Glomerular Causes(Increased glomerulus permeability)Primary Gl

15、omerulonephropathy Minimal Change Disease IgA Nephropathy Idiopathic membranous Glomerulonephritis Focal segmental Glomerulonephritis Membranoproliferative Glomerulonephritis Heavy metals Tubular Causes(Decreased tubular reabsorption)Hypertensive nephrosclerosis Uric Acid nephropathy Acute hypersens

16、itivity Interstitial Nephritis Fanconi Syndrome Heavy metals Sickle Cell Anemia NSAIDs Antibiotics Secondary Glomerulonephropathy Diabetes Mellitus(Diabetic Nephropathy)Systemic Lupus Erythematosus(Lupus Nephritis)Amyloidosis Preeclampsia(Pregnancy Induced Hypertension)Infection HIV Infection Hepati

17、tis B Hepatitis C Poststreptococcal Glomerulonephritis Syphilis Malaria Endocarditis Lung Cancer Gastrointestinal Cancer Lymphoma Renal transplant rejection Overflow Causes(Increased low MW protein production)Hemoglobinuria Myoglobinuria Multiple Myeloma Amyloidosis Glucose in urine Reference value

18、Qualitative method:negative Glycosuria-qualitative test is positive 1.hyperglycemia:diabetes mellitus Cushings syndrom 2.without hyperglycemia:renal tubular dysfunction,such as pyelonephritis Ketones in urine Including three ketone bodies:acetoacetic acid 20%acetone 2%-hydroxybutyric acid 78%The products of fat metabolism Reference value:qualitative method:negative Ketonuria-qualitative test is positive Ketonuria 1.diabetic ketonuria 2.nondiabetic ketonuria:Hyperemesis of pregnancy Patients accompanied by vomiting or diarrhea

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