1、Autoclave挑菌，培養基選擇Overnight cultureShaking bath 培養分光光度計測量細菌濃度數據分析生物工程的實驗要求精確，稍一點污染便會導致失敗，來自外界無數看不見的菌類，都可能在操作時造成污染，為確保實驗儀器、設備無菌，在使用前必須先加以滅菌。溫度-120125壓力-2 atm Fig.11.1Fig.11.2aFig.11.2dFig.11.12Determining cell mass dry weightoptical density(O.D.)spectrophotometerO.D.550 O.D.600Fig.7.17Fig.7.18Fig.7.19
2、Time(hr)OD值05.00E-0211.80E-011.52.81E-0125.38E-012.59.50E-0131.37E+003.52.03E+0042.27E+004.52.68E+0053.06E+005.53.05E+0063.12E+006.53.13E+0073.18E+007.53.17E+0083.18E+00maxGrowth curve of cellTime(hr)dtdxx1maxwhen is constant txx0lnln由由plot log OD vs.tThe greatest slope in linear region:由實驗數據得知由實驗數據得知:1max9316.023538.0ln366.1lnhrhr.(1).(2)Maximum growth ratedoubling time of cellsdtxx)ln(0.(3)2lnd9316.02lndhrd744.0min6.44d2ln.(4)occurs when d02xx
