1、血涂片制备染色及血涂片制备染色及白细胞形态观察白细胞形态观察临床检验基础与输血教研室根据不同的细胞种类及细胞的不同成分，对酸性及碱性染料的结合能力不同，而使各种细胞呈现出各自的特点。掌握血涂片的制备和染色方法及白细胞的正常形态原理原理 目的目的器材器材 载玻片、推片、洗耳球、显微镜、微量吸管、香柏油、乙醇-乙醚溶液、拭镜纸试剂试剂 瑞氏染液、磷酸缓冲液标本标本 抗凝血血血 涂涂 片制片制 备备推片载玻片血液将推片匀速向前，勿停顿。涂片的厚薄取决于速度和角度：快、大(厚)。慢、小（薄）一张满意的血涂片应厚薄均匀 头 体 尾鲜明涂片干燥后用铅笔在血涂片的头部写上姓名和编号李四B 1 2 3血血 涂
2、涂 片片 染色染色勿冲洗再加勿冲洗再加瑞氏染色瑞氏染色液液5-8滴后将滴后将液和液和液充分混匀静置液充分混匀静置10分钟分钟直接流水冲洗3-5分钟（切勿先倒掉染液）涂片经水洗干燥后用油镜分类计数100个白细胞李四李四123123涂片干燥后加瑞氏染色涂片干燥后加瑞氏染色液液5-8滴滴覆盖整个血膜覆盖整个血膜1分钟分钟血涂片的观察血涂片的观察低倍镜观察低倍镜观察1.1.染色情况染色情况(满意、偏酸、偏碱）满意、偏酸、偏碱）2.2.细胞分布情况细胞分布情况油镜下分类计数：体尾交界处。油镜下分类计数：体尾交界处。油镜分类时应按一定走向，不要重复计数油镜分类时应按一定走向，不要重复计数。李四李四1231
3、23 中性杆状核粒细胞 中性分叶核粒细胞 嗜酸性粒细胞 嗜碱性粒细胞 单核细胞1.本次实验的关键环节是什么？2.每人分类的结果与本组其他人使用同一标本的同学是否一致，分析原因。分析讨论分析讨论K+oN3sR6vVazYdC$hG-nN2rQ5vU9yXcC!gF&jJ-nM1qQ5uT8yXcB#fF&jI)mM1qP4uT8xWbB#fE%iI)mL0pP4tS7xWbAZeE%iH(lL0pO3tS7wVaAZeD$hH(lK+oO3sR6wVazYdD$hG*kK+oN2rR6vU9zYdC!gG*kJ-nN2rQ5vU9yXcC!gF&jJ-nM1qQ5uT8yXcB#fF&jI)mM
4、1qP4uT8xWbB#fE%iI)mL0pP4tS7xWbAZeE%iH(lL0pO3sS7wVaAZeD$hH(lK+oO3sR6wVazYdD$hG*kK+oN2rR6vU9zYdC!gG*kJ-nN2rQ5vU9yXcC!gF&jJ-nM1qQ5uT8yXcB#fF&jI)mM1qP4uT8xWbB#fE%iI)mL0pP4tS7xWbAZeE%iH(lL0pO3sS7wVaAZeD$hH(lK+oO3sR6wVazYdD$hG*kK+oN2rR6vU9zYdC!gG*kJ-nN2rQ5vU9yXcC!gF&jJ-nM1qQ5uT8yXcB#fF&jI)mM1qP4tT8xWbB#fE
5、%iI)mL0pP4tS7xWbAZeE%iH(lL0pO3sS7wVaAZeD$hH(lK+oO3sR6wVazYdD$hG*kK+oN2rR6vU9zYdC!gG*kJ-nN2rQ5uU9yXcC!gF&jJ-nM1qQ5uT8yXcB#fF&jI)mM1qP4tT8xWbB#fE%iI)mL0pP4tS7xWbAZeE%iH(lL0pO3sS7wVaAZeD$hH(lK+oO3sR6wVazYdD$hG*kK+oN2rR6vU9zYdC!gG*kJ-nN2rQ5uU9yXcC!gF&jJ-nM1qQ5uT8yXcB#fF&jI)mM1qP4tT8xWbB#fE%iI)mL0pP4tS7x
6、WbAZeE%iH(lL0pO3sS7wVaAZeD$hH(lK+oO3sR6vVazYdD$hG*kK+oN2rR6vU9zYdC!gG*kJ-nN2rQ5uU9yXcC!gF&jJ-nM1qQ5uT8yXcB#fF&jI)mM1qP4tT8xWbB#fE%iI)mL0pP4tS7wWbAZeE%iH(lL0pO3sS7wVaAZeD$hH(lK+oO3sR6vVazYdD$hG*kK+oN2rR6vU9zYdC!gG*kJ-nN2rQ5uU9yXcC!gF&jJ-nM1qQ5uT8yXcB#fF&jI)mM1qP4tT8xWbB#fE%iI)mL0pP4tS7wWbAZeE%iH(lL0p
7、O3sS7wVaAZeD$hH(lK+oO3sR6vVazYdD$hG*kK+oN2rR6vU9zYdC!gG*kJ-nN2rQ5uU9yXcC!gF&jJ-nM1qQ5uT8xXcB#fF&jI)mM1qP4tT8AZfE%iH(mL0pO3tS7wVbAZeD$iH(lK+pO3sR7wVazYeD$hG*lK+oN2sR6vUazYdC!hG*kJ-oN2rQ5vU9yXdC!gF&kJ-nM1rQ5uT9yXcB#gF&jI)nM1qP4uT8xWcB#fE%jI)mL0qP4tS8xWbAZfE%iH(mL0pO3tS7wVbAZeD$iH(lK+pO3sR6wVazYeD$hG*l
8、K+oN2sR6vUazYdC!hG*kJ-oN2rQ5vU9yXdC!gF&kJ-nM1rQ5uT9yXcB#gF&jI)nM1qP4uT8xWcB#fE%jI)mL0qP4tS7xWbAZfE%iH(mL0pO3tS7wVbAZeD$iH(lK+pO3sR6wVazYeD$hG*lK+oN2sR6vUazYdC!hG*kJ-oN2rQ5vU9yXdC!gF&kJ-nM1rQ5uT9yXcB#gF&jI)nM1qP4uT8xWcB#fE%jI)mL0qP4tS7xWbAZfE%iH(mL0pO3tS7wVbAZeD$iH(lK+pO3sR6wVazYeD$hG*lK+oN2sR6vUazYd
9、C!hG*kJ-oN2rQ5vU9yXdC!gF&kJ-nM1rQ5uT8yXcB#gF&jI)nM1qP4uT8xWcB#fE%jI)mL0qP4tS7xWbAZfE%iH(mL0pO3tS7wVbAZeD$iH(lK+pO3sR6wVazYeD$hG*lK+oN2sR6vU9zYdC!hG*kJ-oN2rQ5vU9yXdC!gF&kJ-nM1rQ5uT8yXcB#gF&jI)nM1qP4uT8A#fE%iH)mL0pO4tS7wWbAZeD%iH(lK0pO3sR7wVazZeD$hG(lK+oN3sR6vVazYdC$hG*kJ+oN2rQ6vU9yYdC!gF*kJ-nM2rQ5uU9
10、yXcB!gF&jI-nM1qP5uT8xXcB#fE&jI)mL1qP4tS8xWbA#fE%iH)mL0pO4tS7wWbAZeD%iH(lK0pO3sR7wVazZeD$hG(lK+oN3sR6vVazYdC$hG*kJ+oN2rQ6vU9yYdC!gF*kJ-nM2rQ5uU9yXcB!gF&jI-nM1qP5uT8xXcB#fE&jI)mL1qP4tS8xWbA#fE%iH)mL0pO4tS7wWbAZeD%iH(lK0pO3sR7wVazZeD$hG(lK+oN3sR6vVazYdC$hG*kJ+oN2rQ6vU9yYdC!gF*kJ-nM2rQ5uT9yXcB!gF&jI-nM1
11、qP5uT8xXcB#fE&jL0pP4tS7xWbAZeE%iH(lL0pO3sS7wVaAZeD$hH(lK+oO3sR6wVazYdD$hG*kK+oN2rR6vU9zYdC!gG*kJ-nN2rQ5vU9yXcC!gF&jJ-nM1qQ5uT8yXcB#fF&jI)mM1qP4uT8xWbB#fE%iI)mL0pP4tS7xWbAZeE%iH(lL0pO3sS7wVaAZeD$hH(lK+oO3sR6wVazYdD$hG*kK+oN2rR6vU9zYdC!gG*kJ-nN2rQ5vU9yXcC!gF&jJ-nM1qQ5uT8yXcB#fF&jI)mM1qP4tT8xWbB#fE%iI)
12、mL0pP4tS7xWbAZeE%iH(lL0pO3sS7wVaAZeD$hH(lK+oO3sR6wVazYdD$hG*kK+oN2rR6vU9zYdC!gG*kJ-nN2rQ5uU9yXcC!gF&jJ-nM1qQ5uT8yXcB#fF&jI)mM1qP4tT8xWbB#fE%iI)mL0pP4tS7xWbAZeE%iH(lL0pO3sS7wVaAZeD$hH(lK+oO3sR6wVazYdG*kJ-oN2rQ6vU9yXdC!gF&kJ-nM1rQ5uT9yXcB#gF&jI)nM1qP5uT8xWcB#fE%jI)mL0qP4tS8xWbAZfE%iH(mL0pO3tS7wVbAZeD$
13、iH(lK+pO3sR7wVazYeD$hG*lK+oN2sR6vUazYdC!hG*kJ-oN2rQ6vU9yXdC!gF&kJ-nM1rQ5uT9yXcB#gF&jI)nM1qP5uT8xWcB#fE%jI)mL0qP4tS8xWbAZfE%iH(mL0pO3tS7wVbAZeD$iH(lK+pO3sR7wVazYeD$hG*lK+oN2sR6vUazYdC!hG*kJ-oN2rQ6vU9yXdC!gF&kJ-nM1rQ5uT9yXcB#gF&jI)nM1qP4uT8xWcB#fE%jI)mL0qP4tS8xWbAZfE%iH(mL0pO3tS7wVbA$hG(lK+oN3sR6vVazY
14、dC$hG*kJ+oN2rR6vU9yYdC!gF*kJ-nM2rQ5uU9yXcB!gF&jI-nM1qQ5uT8xXcB#fE&jI)mL1qP4tT8xWbA#fE%iH)mL0pP4tS7wWbAZeD%iH(lK0pO3sS7wVazZeD$hG(lK+oN3sR6vVazYdC$hG*kJ+oN2rR6vU9yYdC!gF*kJ-nM2rQ5uU9yXcB!gF&jI-nM1qQ5uT8xXcB#fE&jI)mL4tS7xWbAZeE%iH(lL0pO3tS7wVaAZeD$hH(lK+pO3sR6wVazYdD$hG*kK+oN2sR6vU9zYdC!gG*kJ-oN2rQ5vU
15、9yXcC!gF&jJ-nM1rQ5uT8yXcB#fF&jI)mM1qP4uT8xWbB#fE%iI)mL0qP4tS7xWbAZeE%iH(lL0pO3tS7wVaAZeD$hH(lK+pO3sR6wVazYdD$hG*kK+oN2sR6vU9zYdC!gG*kJ-nN2rQ5vU9yXcC!gF&jJ-nM1rQ5uT8yXcB#fF&jI)mM1qP4uT8xWbB#fE%iI)mL0qP4tS7xWbAZeE%iH(lL0pO3tS7wVaAZeD$hH(lK+oO3sR6wVazYdD$hG*kK+oN2sR6vU9zYdC!gG*kJ-nN2rQ5vU9y#gF&jI-nM1qP
16、5uT8xWcB#fE%jI)mL1qP4tS8xWbAZfE%iH(mL0pO4tS7wVbAZeD$iH(lK0pO3sR7wVazYeD$hG*lK+oN3sR6vUazYdC!hG*kJ+oN2rQ6vU9yXdC!gF&kJ-nM2rQ5uT9yXcB#gF&jI)nM1qP5uT8xWcB#fE%jI)mL1qP4tS8xWbAZfE%iH(mL0pO4tS7wVbAZeD$iH(lK0pO3sR7wVazYeD$hG*lK+oN3sR6vUazYdC!hG*kJ-oN2rQ6vU9yXdC!gF&kJ-nM2rQ5uT9yXcB#gF&jI)nM1qP5uT8xWcB%iI)mL
17、0pP4tS7wWbAZeD%iH(lL0pO3sS7wVazZeD$hG(lK+oO3sR6vVazYdC$hG*kK+oN2rR6vU9yYdC!gF*kJ-nN2rQ5uU9yXcB!gF&jJ-nM1qQ5uT8xXcB#fE&jI)mM1qP4tT8xWbA#fE%iH)mL0pP4tS7wWbAZeD%iH(lL0pO3sS7wVazZeD$hG(lK+oO3sR6vVazYdC$hG*kK+oN2rR6vU9yYdC!gF*kJ-nN2uT8yXcB#fF&jI)nM1qP4uT8xWbB#fE%jI)mL0qP4tS7xWbAZeE%iH(mL0pO3tS7wVaAZeD$iH
18、(lK+pO3sR6wVazYdD$hG*lK+oN2sR6vU9zYdC!gG*kJ-oN2rQ5vU9yXcC!gF&kJ-nM1rQ5uT8yXcB#fF&jI)nM1qP4uT8xWbB#fE%jI)mL0qP4tS7xWbAZeE%iH(mL0pO7wVazYeD$hG(lK+oN3sR6vUazYdC$hG*kJ+oN2rQ6vU9yXdC!gF*kJ-nM2rQ5uT9yXcB!gF&jI-nM1qP5uT8xWcB#fE&jI)mL1qP4tS8xWbAZfE%iH)mL0pO4tS7wVbAZeD%iH(lK0pO3sR7wVazYeD$hG(lK+oN3sR6vUazYdC$hG*kJ+oN2rQ6vU9yXgF&jJ-nM1qQ5uT8xXcB#fF&jI)mM1qP4tT8xWbB#fE%iI)mL0pP4tS7wWbAZeE%iH(lL0pO3sS7wVaAZeD$hH(lK+oO3sR6vVazYdD$hG*kK+oN2rR6vU9yYdC!gG*kJ-nN2rQ5uU9yXcC!gF&jJ-nM1qQ5uT8xXcB#fF&jI)mM1qP4tT8xWbB#fE%iL0pO3tS7wVbAZeD$iH(lK+pO3sR6wVazYeD$hG*lK+oN2sR6vUazYdC!hG*kM2rQ5uU9yXcB!gF&jI-nM1qP5uT8xXc
