1、本试验主要通过三种工艺1.鲜元胡切片在醋中进行减压抽真空处理,以促使醋充分渗透进元胡片中;2.鲜切片干燥后用醋湿润处理;3.鲜元胡直接在醋中煮一定时间处理。并对各种处理工艺所制的元胡饮片与传统炮制饮片进行对比研究,分析比较两者在有效成分含量、水浸提物和醇浸提物含量、有效成分延胡索乙素溶出度等方面的差异。传统炮制元胡传统炮制元胡元胡片元胡片鲜切片米醋减压鲜切片米醋减压2 2次次鲜切片失部分水后米醋浸鲜切片失部分水后米醋浸传统炮制样品色谱图,其中4号峰为原阿片碱,9号峰为小檗碱,15号峰为延胡索乙素 8批元胡一体化饮片指纹图谱加工炮制方法原阿片碱(mg/g)延胡索乙素(mg/g)传统加工炮制0.3
2、3160.6097鲜切片米醋中减压抽真空2次0.36540.8095鲜切片10%醋酸中减压抽真空2次0.31390.7391鲜切片20%醋酸中减压抽真空2次0.31630.7105鲜切片干燥后用米醋浸润0.36020.7894整个鲜元胡米醋煮10min0.28330.5737整个鲜元胡米醋煮20min0.30540.5321整个鲜元胡米醋煮40min0.27120.5450市购醋元胡片(武林门药店)0.01270.1080市购醋元胡片(古荡药店)0.04120.1046不同加工炮制处理元胡饮片中原阿片碱和延胡索乙素含量加工炮制方法水热浸提物含量(%)稀乙醇热浸提物含量(%)鲜切片米醋中减压抽真
3、空23.2117.72鲜切片干燥后米醋浸润34.4117.38传统炮制21.7416.34元胡样品水浸提物和醇浸提物含量不同样品元胡对小鼠醋酸扭体反应的影响(n=16,XSD)组别扭体潜伏期(s)扭体次数/20min抑制率(%)样品1547.40347.485.334.35*63.3%样品2473.47340.489.338.6235.8%样品3518.13289.1711.477.8521.1%生理盐水组428.13249.2114.5311.981.*P0.01与生理盐水组相比2.样品1-在醋中减压抽真空;样品2-切片干燥后醋浸润;样品3-传统炮制元胡 不同元胡样品对小鼠热板测试舔足反应潜
4、伏期的影响(n=22,XSD)组别基础潜伏期(s)药后各时间段的舔足潜伏期(s)30min60min90min120min样品133.5514.7336.3519.4934.1322.71*41.3018.81*32.3919.57*样品233.7312.4734.4322.5630.8618.68*34.6219.75*30.3815.25*样品333.2317.0129.8615.8119.1011.9230.3824.4521.5720.05生理盐水组33.4512.4227.2716.6915.647.1320.3210.5420.7310.42*P0.05、*P0.01与生理盐水组相比:;P0.05与样品3组相比。不同加工炮制处理白术饮片白术内酯和白术内酯含量谢谢 谢!谢!