1、通过本章学习应掌握以下内容通过本章学习应掌握以下内容：Step1Step2Step3Step4Langmuir cKcqq0Langmuir)1/(ADSADSCKCKOH+CH2OCHCH2ClOCH2CHCH2O+PNH2OCH2NHP表氯醇Activation(ion exchange)DEAE anion exchangerCMC Cation Exchanger nsSAZAZSnsAZAZ22112211111121,AASS,tKF1)1ln(00113rrDK2022122161rtnDnienFK1外扩散速度常数F时间为t时，树脂的饱和度r0为树脂颗粒半径D1为液相中的扩散系
2、数为吸附常数ssBABARABRKR-A、R-B表示结合在树脂上的A离子和B离子浓度AS、BS表示溶液中A离子和B离子ssBAABARBRK/越大B越易被交换BAK Some of the charged regions which will influence ion exchange Different proteins have different charges and different patterns of surface chargeBasis for selectivityNH3RCOOH+NH3RCOO+-RNH2COO-Low pHPositive chargeHigh
3、pHNegative chargeHydrogen gainedHydrogen lostEffect of pH on charge-+Overall charge on proteinNH3RCOOH+NH3RCOO+-RNH2COO-acid isoelectric point alkalineexcess positive charge balanced positive and negative charge excess negative chargepH310Titration curvesIxv_2 JB 98091020Charge on protein-+pH310Anion exchangerCation exchangerControlling selectivity by pH+-anion exchange beadEquilibrationSample applicationand wash-+-Elution-+-Regeneration-+Sampleapplicationand washElutionEquilibrationRegeneration-+anionexchangerbead-
