1、Chapter 10 Recombination/重组重组10.1 Homologous Recombination 10.1.1 Mechanism for Crossing-Over 10.1.2 Mechanism for Double-Stranded Break Repair 10.1.3 The RecBCD pathway 10.1.4 Gene Conversion10.2 Non-homologous Recombination 10.2.1 Transposons 10.2.2 Retrotransposons 10.2.3 Bacteriophage integratio
2、n10.1 同源重组同源重组 10.1.1 交换机理交换机理 10.1.2 双链断裂双链断裂 修复机理修复机理 10.1.3 RecBCD途径途径 10.1.4 基因转换基因转换10.2 非同源重组非同源重组 10.2.1 转座子转座子 10.2.2 反转录转座子反转录转座子 10.2.3 噬菌体的整合噬菌体的整合10.1 Homologous Recombination 同源重组同源重组Diploid(2n)2 copies of eachchromosomeHomologous RecombinationIf these chromosomes were left unchanged Ch
3、ildGrand-childIdenticalCrossing-over in meiosisChildGrand-childDifferentHomologous Recombination in DNA repairHomologouschromosomes10.1.1 Mechanism for Crossing-Over /交换机理交换机理Mechanism of recombination in yeast(1/2)Mechanism of recombination in yeast(2/2)Holliday junctionsBranch migration of Hollida
4、y junctionsHolliday交叉的分支迁移交叉的分支迁移Resolution of the Holliday junctionHolliday交叉的拆分交叉的拆分Resolution of the Holliday junctionto create a crossover产生交换的产生交换的Holliday交叉拆分交叉拆分Resolution of Holliday junctionsfor non-crossover不产生交换不产生交换的的Holliday交叉拆分交叉拆分Resolution of the Holliday junctionto create a crossove
5、r产生交换的产生交换的Holliday交叉拆分交叉拆分10.1.2 Mechanism for Double-Stranded Break Repair /双链断裂修复机理双链断裂修复机理Resolution of Holliday junctionsfor non-crossover不产生交换的不产生交换的Holliday交叉拆分交叉拆分Brca1/Brca2 deletion and breast cancer Brca1/Brca2缺失与乳腺癌缺失与乳腺癌www.harthosp.org/cancer/breast.htm The roles of BRCA1 and BRCA2 in
6、DNA repair BRCA1和和BRCA2在在DNA修复中的作用修复中的作用www-ermm.cbcu.cam.ac.uk/01003118h.htm Now showingMeselson-Radding Heteroduplex ModelFile:biophoto7 Introduction to Genetic Analysis Meselson-Radding heteroduplex Model10.1.3 The RecBCD Pathway (1/3)The RecBCD Pathway(2/3)The RecBCD Pathway(3/3)10.1.4 Gene Conv
7、ersion /基因转换基因转换G GT TA AT TG GC CA AT THomologous,Not identical.35353535Gene Conversion /基因转换基因转换Gene Conversion /基因转换基因转换Neurospora crassa 粗糙脉孢菌粗糙脉孢菌+mmMeiosis I减数分裂减数分裂IMeiosis II减数分裂减数分裂IIMitosis有丝分裂有丝分裂+mm+mmmmmm+4:4Neurospora crassa 粗糙脉孢菌粗糙脉孢菌www.nigms.nih.gov/Initiatives/Models/www.neurospora
8、genome.org/www.neurosporagenome.org/4:45:36:2Now showingGene ConversionFile:biophoto7 Introduction to Genetic Analysis Genetic Consequences of Meselson-Radding Model10.2 Non-homologous Recombination非同源重组非同源重组10.2.1 Transposons 转座子转座子10.2.2 Retrotransposons 反转录转座子反转录转座子10.2.3 Bacteriophage integratio
9、n 噬菌体的整合噬菌体的整合10.2.1 Transposons /转座子转座子Transposition /转座转座Generation of direct repeats同向重复序列的产生同向重复序列的产生Now showingReplicative transpositionFile:biophoto7 Introduction to Genetic Analysis Replicative transposition.(5)10.2.2 Retrotransposons 反转录转座子反转录转座子LTR-RetrotransposonsLTR反转录转座子反转录转座子Non-LTR Ret
10、rotransposons非非LTR反转录转座子反转录转座子LTR-RetrotransposonsLTR反转录转座子反转录转座子Non-LTR retrotransposons非非LTR反转录转座子反转录转座子Weavers Molecular Biology,3rd Edition,Figure 23.3110.2.3 Bacteriophage integration噬菌体的整合噬菌体的整合LysisLysogenyPhage integration and excisionattP:attachment sites of phageattB:attachment sites of bacterium
