1、Experiment for Pharmaceutics of TCM,For grade 3 , semester 2,China Pharmaceutical University,Teacher：Wei Yuanfeng weiyuanfengyuer,China Pharmaceutical University,Exercise 1 Cyclodextrin Inclusion Complexes,Peppermint Oil -CD Complex,6,7,8,China Pharmaceutical University,Introduction,What are Cyclode
2、xtrins (CDs)?,China Pharmaceutical University,What is the structure of the -CD?,-CD are molecular capsules with a hydrophobic inside and a hydrophilic outside.,-OH,-O-,-OH,Introduction,China Pharmaceutical University,What are the inclusion complexes?,Molecules of appropriate size and shape fit into
3、the CD cavity to form the inclusion complexes.,Guest molecules,Host molecules,Solid molecules,Liquid molecules,Gaseous molecules,Introduction,China Pharmaceutical University,How to produce cyclodextrin inclusion complexes?,(1) Saturated water solution / Co-precipitation / Re-crystallization Method,(
4、2) Grinding / kneading Method,(3) Freeze-drying Method,(4) Spray drying Method,(5) Ultrasonic Method,Introduction,-CD 8 g Inclusion Compound Peppermint oil 2 mL API Water 16 mL solvent,China Pharmaceutical University,Formulation,-CD+16 mL water add peppermint oil vacuum filtration Wash the residue w
5、ith 5ml 95% ethanol Dry the inclusion complexes at 40 Store the product in a desiccator,Mixing,Grinding for 30 min,China Pharmaceutical University,Section 1: Process,peppermint oil 2 mL,95% Ethanol 2 mL,+,China Pharmaceutical University,Identification Method,How to identify the cyclodextrin inclusio
6、n complexes?,(1) x-ray diffractometry,(2)Differential thermal analysis/ Differential scanning calorimetry,(3) Microscopic examination,(4) Fluorescent Spectrometry,(5)TLC,(6)UV, IR, NMR, HPLC, GC, et al,1. Solution preparation The sample: 0.5 g inclusion complexes + 2mL 95% ethanol ultrasonication fo
7、r 30min the supernatant as the test solution The reference : 2d peppermint oil + 2mL 95% ethanol 2. TLC Identification Apply separately to the plate 2L of the sample solution and reference solution. Developing solvent: acetyl-petroleum (15:85,V/V) Color developing: dry the plate in air, spray with a
8、 1% solution of vanillaldehyde in sulphuric acid and heat until the spots are distinct.,China Pharmaceutical University,Section 2: Identification,China Pharmaceutical University,Exercise 2: Ointment,Unguentum Geranii (Common Herons Bill Ointment),China Pharmaceutical University,What are ointments?,O
9、intments are paste-like preparations with a suitable consistency, usually containing medicaments, crude drugs and their extracts incorporated in suitable base, and intended for external application to the skin.,The types of the ointments,(1) Fatty bases,(2) Water soluble bases,(3) Emulsifying bases,
10、Introduction,Herba Erodii seu Geranii 50g API Wool fat 2.5g base Ethyl p-hydroxybenzoate 0.015g (2d) preservative Vaseline Q.S base Make up to 50g,China Pharmaceutical University,Formulation,Section 1: Extraction Herba Erodii seu Geranii extract 50ml Stand for 7d supernatant Concentrate to 3-4g Section 2: Ointment Preparation The extract + Ethyl p-hydroxybenzoate + Wool fat +Vaseline mix thoroughly and the ointment is completed,300ml，30min,500ml，40min,concentrate,Add 50mL 95% ethanol,vaccum filtrate,China Pharmaceutical University,Process,to,
