续前理理nLH/22212)(16)(54. 5)(WtWttnRRR理effeffnLH/2212)(54. 5)(16WtWtnRReff0ttkR2)1(kknneff理 (填充柱):uCuBAHGC/(毛细管柱)或uCuBH/dpA2dpAgmDDB22gRDBtB,MTDTDgg或llgsmCCCCCCllDdfC2TDL续前uCAHHPLC:mDB2TDm相忽略大低,流动相柱温BTRtnHuuHmlu,但柱效,时,min/1min/1mlu 选择兼顾柱效和分析时间,dpA2dpA柱效,nHAdp图示续前)(忽略固定相传质阻抗smmssmmCCCCCC忽略固定相传质阻抗态流动相的传质阻抗注:只考虑流动相和静uCuCAHHPLCsmm:msmmDdpCC2mDdpC2TDm柱效,nHCdp柱效,nHDm,柱阻,但易产生气泡mmDTCDT图示 )1 (0mRVSKttmVSKk续前续前续前续前22114kknRuCAH流动相采用粘度小、低流速的匀的固定相:采用粒度小、填充均n12121212RRRRVVttkkKK温影响小)质和固定相性质(受柱:调整流动相种类、性0ttVVKVCVCWWkRmsmmssms小)的配比(受柱温影响较:调整流动相和固定相k续前续前 图示续前