1、颅内淋巴瘤大剂量mtx化疗1 甲氨蝶呤（甲氨蝶呤（methotrexate,MTX）属抗代谢药，化学结构与叶酸相似，对二氢叶酸还原酶有强大而持久的抑制作用，干扰核酸的生物合成，特异性作用于S期，阻止肿瘤细胞的分裂繁殖，为细胞周期特异性药物。颅内淋巴瘤大剂量mtx化疗2还原性叶酸载体（reduced folate carrier,RFC）甲氨蝶呤聚谷氨酸合成酶（folylpolyglutamate synthetase,FPGS）MTX多聚谷氨酸（methotrexate polyglutamine，MTXPG）谷氨酸水解酶（gammaglutamylhydtrolase，GGH）胞外胞内颅内淋
2、巴瘤大剂量mtx化疗3，颅内淋巴瘤大剂量mtx化疗4颅内淋巴瘤大剂量mtx化疗5颅内淋巴瘤大剂量mtx化疗6颅内淋巴瘤大剂量mtx化疗7颅内淋巴瘤大剂量mtx化疗8颅内淋巴瘤大剂量mtx化疗9颅内淋巴瘤大剂量mtx化疗10MTXMTXMTXMTXR RF FC C MTX20umol/LMTX20umol/LMTXPGMTXPGGGHGGH还原性叶酸载体（reduced folate carrier,RFC）甲氨蝶呤聚谷氨酸合成酶（folylpolyglutamate synthetase,FPGS）MTX多聚谷氨酸（methotrexate polyglutamine，MTXPG）谷氨酸水解
3、酶（gammaglutamylhydtrolase，GGH）FPGSFPGSGluGlu (27)(27)颅内淋巴瘤大剂量mtx化疗11颅内淋巴瘤大剂量mtx化疗12MTXMTXMTXMTXR RF FC C MTX20umol/LMTX20umol/LMTXPGMTXPGGGHGGH还原性叶酸载体（reduced folate carrier,RFC）甲氨蝶呤聚谷氨酸合成酶（folylpolyglutamate synthetase,FPGS）MTX多聚谷氨酸（methotrexate polyglutamine，MTXPG）谷氨酸水解酶（gammaglutamylhydtrolase，GG
4、H）FPGSFPGSGluGlu (27)(27)颅内淋巴瘤大剂量mtx化疗13 J Clin Invest,1985,75(3):1008 1014 J Biol Chem,1985,260(117):9720 9726 J Clin Invest,1994,94:1996-2001颅内淋巴瘤大剂量mtx化疗14颅内淋巴瘤大剂量mtx化疗15 颅内淋巴瘤大剂量mtx化疗16 颅内淋巴瘤大剂量mtx化疗17颅内淋巴瘤大剂量mtx化疗18颅内淋巴瘤大剂量mtx化疗19颅内淋巴瘤大剂量mtx化疗20。颅内淋巴瘤大剂量mtx化疗21 颅内淋巴瘤大剂量mtx化疗22颅内淋巴瘤大剂量mtx化疗23颅内淋
5、巴瘤大剂量mtx化疗24颅内淋巴瘤大剂量mtx化疗25颅内淋巴瘤大剂量mtx化疗26颅内淋巴瘤大剂量mtx化疗27颅内淋巴瘤大剂量mtx化疗28 颅内淋巴瘤大剂量mtx化疗29MTXMTXMTXMTXR RF FC C MTX20umol/LMTX20umol/LMTXPGMTXPGGGHGGH还原性叶酸载体（reduced folate carrier,RFC）甲氨蝶呤聚谷氨酸合成酶（folylpolyglutamate synthetase,FPGS）MTX多聚谷氨酸（methotrexate polyglutamine，MTXPG）谷氨酸水解酶（gammaglutamylhydtrolase，GGH）FPGSFPGSGluGlu (27)(27)颅内淋巴瘤大剂量mtx化疗30 颅内淋巴瘤大剂量mtx化疗31颅内淋巴瘤大剂量mtx化疗32颅内淋巴瘤大剂量mtx化疗33颅内淋巴瘤大剂量mtx化疗34
